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Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956]

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Catalog# ET1702-06

Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956]

Application

  • WB

  • IP

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

This product has been cited in peer reviewed publications, see list HERE

概述

产品名称

Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956]

抗体类型

Recombinant Rabbit monoclonal Antibody

免疫原

Synthetic peptide within Human CD71 aa 22-60 / 760.

种属反应性

Human, Mouse, Rat

验证应用

WB, IP, FC

分子量

Predicted band size: 85 kDa

阳性对照

HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.

偶联

unconjugated

克隆号

JF0956

RRID

AB_3070270

产品特性

形态

Liquid

浓度

1ug/ul

存放说明

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

存储缓冲液

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

亚型

IgG

纯化方式

Protein A affinity purified.

应用稀释度

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:50-1:100

  • IP

  • Use at an assay dependent concentration.

发表文章中的应用

WB See 12 publications below
IHC See 1 publications below

发表文章中的种属

Mouse See 6 publications below
Human See 5 publications below
Porcine See 1 publications below

靶点

功能

CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.

背景文献

1. Wang Y et al. Role of hemoglobin and transferrin in multi-wall carbon nanotube-induced mesothelial injury and carcinogenesis. Cancer Sci 107:250-7 (2016).

2. Song J et al. Native low density lipoprotein promotes lipid raft formation in macrophages. Mol Med Rep 13:2087-93 (2016).

序列相似性

Belongs to the peptidase M28 family. M28B subfamily.

翻译后修饰

N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.

亚细胞定位

Cell membrane, Melanosome, Secreted.

UNIPROT #

SwissProt: P02786 Human

SwissProt: Q62351 Mouse

SwissProt: Q99376 Rat

别名

CD 71 antibody

CD71 antibody

CD71 antigen antibody

IMD46 antibody

OTTHUMP00000208523 antibody

OTTHUMP00000208524 antibody

OTTHUMP00000208525 antibody

p90 antibody

sTfR antibody

T9 antibody

展开

图片

  • Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/2,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: K-562 cell lysate<br />Lane 3: SW480 cell lysate<br />Lane 4: U-87 MG cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: Mouse spleen tissue lysate<br />Lane 7: Rat spleen tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 5 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/2,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: K-562 cell lysate
    Lane 3: SW480 cell lysate
    Lane 4: U-87 MG cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: Mouse spleen tissue lysate
    Lane 7: Rat spleen tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 5 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa-si NT cell lysate<br />Lane 2: HeLa-si Transferrin Receptor cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 1 minute 20 seconds;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/1,000 dilution.

    Lane 1: HeLa-si NT cell lysate
    Lane 2: HeLa-si Transferrin Receptor cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 100 kDa

    Exposure time: 1 minute 20 seconds;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/5,000 dilution.<br /><br />Lane 1: HAP1 WT cell lysate<br />Lane 2: HAP1 Transferrin Receptor KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 85 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 1 minute 48 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Transferrin Receptor (CD71) on different lysates with Rabbit anti-Transferrin Receptor (CD71) antibody (ET1702-06) at 1/5,000 dilution.

    Lane 1: HAP1 WT cell lysate
    Lane 2: HAP1 Transferrin Receptor KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 85 kDa
    Observed band size: 90 kDa

    Exposure time: 1 minute 48 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/5,000 dilution was used in TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71).<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/ET1702-06" style="font-weight: bold;text-decoration: underline;">ET1702-06</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HeLa cells labeling Transferrin Receptor (CD71).

    Cells were fixed and permeabilized. Then stained with the primary antibody (ET1702-06, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

引文

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  2. IHC
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