IL-6R Rabbit Polyclonal Antibody
Catalog# HA500438
IL-6R Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Human
概述
产品名称
IL-6R Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Synthetic peptide within human IL-6R aa 20-69/468.
种属反应性
Human
验证应用
WB, IF-Cell, IHC-P
分子量
Predicted band size: 52 kDa
阳性对照
Daudi cell lysate, A549 cell lysate, K562 cell lysate, Hela, human fetal skeletal muscle tissue.
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:1,000
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IF-Cell
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1:200
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IHC-P
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1:600
靶点
功能
This gene encodes a subunit of the interleukin 6 (IL6) receptor complex. Interleukin 6 is a potent pleiotropic cytokine that regulates cell growth and differentiation and plays an important role in the immune response. The IL6 receptor is a protein complex consisting of this protein and interleukin 6 signal transducer (IL6ST/GP130/IL6-beta), a receptor subunit also shared by many other cytokines. Dysregulated production of IL6 and this receptor are implicated in the pathogenesis of many diseases, such as multiple myeloma, autoimmune diseases and prostate cancer. Alternatively spliced transcript variants encoding distinct isoforms have been reported. A pseudogene of this gene is found on chromosome 9.
背景文献
1. Bin S. et. al. Targeting miR-10a-5p/IL-6R axis for reducing IL-6-induced cartilage cell ferroptosis. Exp Mol Pathol. 2021 Feb
2. Zongfei J. et. al. In vitro IL-6/IL-6R Trans-Signaling in Fibroblasts Releases Cytokines That May Be Linked to the Pathogenesis of IgG4-Related Disease. Front Immunol. 2020 Jul
亚细胞定位
Cell membrane, Secreted.
UNIPROT
别名
CD 126 antibody
CD126 antibody
CD126 antigen antibody
gp80 antibody
IL 6R 1 antibody
IL 6R alpha antibody
IL 6R antibody
IL-6 receptor alpha chain antibody
IL-6 receptor subunit alpha antibody
IL-6R 1 antibody
展开图片
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Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA500438) at 1/1,000 dilution.
Lane 1: Daudi cell lysate
Lane 2: A549 cell lysate
Lane 3: K562 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 47 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500438) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
ICC staining of IL-6R in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (HA500438, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-IL-6R antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500438, 1/600) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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