CD47 Recombinant Rabbit Monoclonal Antibody [PD00-33]
Catalog# HA721158
CD47 Recombinant Rabbit Monoclonal Antibody [PD00-33]
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IHC-P
-
FC
-
Human
概述
产品名称
CD47 Recombinant Rabbit Monoclonal Antibody [PD00-33]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Human CD47 aa 50-150 (internal sequence).
种属反应性
Human
验证应用
IHC-P, FC
分子量
Predicted band size: 35 kDa
阳性对照
Human ovarian tumor tissue, human prostate tissue, Jurkat.
偶联
unconjugated
克隆号
PD00-33
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
IHC-P
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1:1,000
-
FC
-
1:500-1:1,000
靶点
功能
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα). Broad specificity aminopeptidase which plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. Also involved in the processing of various peptides including peptide hormones, such as angiotensin III and IV, neuropeptides, and chemokines. May also be involved the cleavage of peptides bound to major histocompatibility complex class II molecules of antigen presenting cells. May have a role in angiogenesis and promote cholesterol crystallization. CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is ubiquitously expressed in human cells and has been found to be overexpressed in many different tumor cells. Expression in equine cutaneous tumors has been reported as well.
背景文献
1. Zhang W. et. al. Advances in Anti-Tumor Treatments Targeting the CD47/SIRPα Axis. Front Immunol. 2020 Jan
2. Hayat SMG. et. al. CD47: role in the immune system and application to cancer therapy. Cell Oncol (Dordr). 2020 Feb
亚细胞定位
Cell membrane.
UNIPROT
别名
Antigen identified by monoclonal antibody 1D8 antibody
Antigenic surface determinant protein OA3 antibody
CD 47 antibody
CD47 antibody
CD47 antigen (Rh-related antigen, integrin-associated signal transducer) antibody
CD47 antigen antibody
CD47 glycoprotein antibody
CD47 molecule antibody
CD47_HUMAN antibody
IAP antibody
展开图片
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Immunohistochemical analysis of paraffin-embedded human ovarian tumor tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate tissue with Rabbit anti-CD47 antibody (HA721158) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721158) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Jurkat cells labeling CD47.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721158, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
同靶点 & 同通路的产品
CD47 Mouse Monoclonal Antibody [1A9]
Application: IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
