• 概述

• 产品描述 The SuperSignal ECL Western Blotting Substrate is a highly sensitive nonradioactive, enhanced luminol-based chemiluminescent substrate for the detection of horseradish peroxidase (HRP) on immunoblots. The SuperSignal ECL Western Blotting Substrate enables the detection of picogram amounts of antigen and allows for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to X-ray film to obtain optimal results or stripped of the immunodetection reagents and re-probed.
• 产品名称 超敏ECL化学发光试剂盒
• 验证应用 WB,ELISA
• 抗体类型 其它
• 偶联 Non-conjugated

• 性能

• 存放说明 Upon receipt, SuperSignal ECL Western Blotting Substrate can be stored in the dark at 4°C for up to 12 months.
• 其它名称
  • SuperSignal ECL Western Blotting Substrate

• 应用

• 

  Fig1: Detection of beta-actin in HeLa Cell Lysates Visualized with SuperSignal ECL Western Blotting Substrate. A two-fold dilution series of HeLa whole cell lysate was prepared and loaded at 1000, 500, 125, 63, 31, 16, 8, 4, 2 and 0 ng per lane. Samples were transferred onto PVDF membrane and probed with 1 ug/mL of Mouse Anti- beta-actin Monoclonal Antibody (Huabio, Catalog # EM21002) followed by HRP-conjugated Goat anti-Mouse IgG Secondary Antibody (Huabio, Catalog # HA1006). SuperSignal ECL Western Blotting Substrate reagent was applied for 3 minute. Lanes are visualized on 1 minute exposure.

  

  Fig2: Detection of Recombinant Human PTP1B Visualized with SuperSignal ECL Western Blotting Substrate. A two-fold dilution series of HeLa whole cell lysate was prepared and loaded at 800, 400, 200, 100, 50, 25, 12.5, 6.25, 3.1 and 1.55 pg per lane. Samples were transferred onto PVDF membrane and probed with 0.8 ug/mL of Mouse Anti- PTP1B Monoclonal Antibody (Huabio, Catalog # M1511-7) followed by HRP-conjugated Goat anti-Mouse IgG Secondary Antibody (Huabio, Catalog # HA1006). SuperSignal ECL Western Blotting Substrate reagent was applied for 3 minute. Lanes are visualized on 1 minute exposure.

• 背景文献

• 暂无

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.