SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15]
Catalog# HA721627
SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15]
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WB
-
IHC-P
-
FC
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Human
-
Monkey
概述
产品名称
SMARCA2 Recombinant Rabbit Monoclonal Antibody [PSH01-15]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within human SMARCA2 aa 231-280 / 1,590.
种属反应性
Human, Monkey
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 181 kDa
阳性对照
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, MCF7 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human prostate carcinoma tissue, MCF7.
偶联
unconjugated
克隆号
PSH01-15
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:1,000
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IHC-P
-
1:200
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FC
-
1:1,000
靶点
功能
Probable global transcription activator SNF2L2 is a protein that in humans is encoded by the SMARCA2 gene. The protein encoded by this gene is a member of the SWI/SNF family of proteins and is highly similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. Two transcript variants encoding different isoforms have been found for this gene, which contains a trinucleotide repeat (CAG) length polymorphism.
背景文献
1. Cantley J et al. Selective PROTAC-mediated degradation of SMARCA2 is efficacious in SMARCA4 mutant cancers. Nat Commun. 2022 Nov
2. Kofink C et al. A selective and orally bioavailable VHL-recruiting PROTAC achieves SMARCA2 degradation in vivo. Nat Commun. 2022 Oct
亚细胞定位
Nucleus.
UNIPROT
别名
ATP dependent helicase SMARCA2 antibody
ATP-dependent helicase SMARCA2 antibody
BAF190 antibody
BAF190B antibody
BRG1-associated factor 190B antibody
BRM antibody
FLJ36757 antibody
Global transcription activator homologous sequence antibody
hBRM antibody
hSNF2a antibody
展开图片
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☑ Relative expression (RE)
Western blot analysis of SMARCA2 on different lysates with Rabbit anti-SMARCA2 antibody (HA721627) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: A549 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: COS-1 cell lysate
Lane 6: NCCIT cell lysate (low expression)
Lysates/proteins at 30 µg/Lane.
Predicted band size: 181 kDa
Observed band size: 200 kDa
Exposure time: 3 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721627) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA721627) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721627) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue with Rabbit anti-SMARCA2 antibody (HA721627) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721627) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of MCF7 cells labeling SMARCA2.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721627, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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