• 概述

• 产品描述 The nucleocapsid protein (N-protein) is a structural protein that binds to the coronavirus RNA genome, thus creating a shell (or capsid) around the enclosed nucleic acid. The N-protein also interacts with the viral membrane protein during viral assembly, assists in RNA synthesis and folding, plays a role in virus budding, and affects host cell responses, including cell cycle and translation. Coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
• 产品名称 Anti-SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody [A4G9]
• 分子量 46 kDa
• 种属反应性 SARS-CoV-2
• 验证应用 WB,ELISA(Det)
• 抗体类型 小鼠单抗
• 免疫原 Recombinant full length protein corresponding to SARS-CoV-2 nucleocapsid protein aa 1-419.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 2 mg/ml.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH 7.4).
• 亚型 IgG1
• 纯化方式 Protein G affinity purified.
• 亚细胞定位 Virion.
• 数据链接 GenBank: QHD43416.1 SARS-CoV-2
  SwissProt: P0DTC9 SARS-CoV-2
  
• 其它名称
  • 2019-nCoV nucleocapsid protein antibody
  • N antibody
  • NC antibody
  • Nucleoprotein antibody
  • Protein N antibody
  • SARS-CoV-2Nucleocapsidprotein
  more

• 应用

  WB: 0.5-2ug/ml
  ELISA(Det): 10ng/ml

• 

  Fig1: The binding activity of HA600005 with Recombinant SARS-CoV-2 Nucleocapsid protein.
  Immobilized Recombinant SARS-CoV-2 nucleocapsid protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600005) for 1 hour at 25℃. The EC50 of HA600005 is 47.22 ng/ml.

  

  Fig2: This antibody will detect SARS-CoV-2 Nucleocapsid protein in ELISA pair set (Clone: # A4G9-HRP). In a sandwich ELISA, it can be used as Detect antibody when paired with (Cat: # HA600007).

• 背景文献

• 1. Fan Wu. et. al. A new coronavirus associated with human respiratory disease in China. Nature. 2020 Mar;579(7798):265-269.

• Publishing research using Anti-SARS-CoV-2 Nucleocapsid protein Mouse Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.