RNF12 Rabbit Polyclonal Antibody
Catalog# ER1915-89
RNF12 Rabbit Polyclonal Antibody
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WB
-
Human
-
Mouse
概述
产品名称
RNF12 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human RNF12 525-624/624
种属反应性
Human, Mouse
验证应用
WB
分子量
69 KDa
阳性对照
Expressed in many tissues.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:500-2000
靶点
功能
E3 ubiquitin-protein ligase. Acts as a negative coregulator for LIM homeodomain transcription factors by mediating the ubiquitination and subsequent degradation of LIM cofactors LDB1 and LDB2 and by mediating the recruitment the SIN3a/histone deacetylase corepressor complex. Ubiquitination and degradation of LIM cofactors LDB1 and LDB2 allows DNA-bound LIM homeodomain transcription factors to interact with other protein partners such as RLIM. Plays a role in telomere length-mediated growth suppression by mediating the ubiquitination and degradation of TERF1. By targeting ZFP42 for degradation, acts as an activator of random inactivation of X chromosome in the embryo, a stochastic process in which one X chromosome is inactivated to minimize sex-related dosage differences of X-encoded genes in somatic cells of female placental mammals.
背景文献
暂无
亚细胞定位
Cytoplasmic and Nuclear
UNIPROT
别名
RLIM
RING finger LIM domain-binding protein
Ring finger protein 12
RNF12_HUMAN.
图片
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Sample:
placenta (Mouse) Lysate at 40 ug
Primary: Anti-RNF12 (ER1915-89) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD -
Sample:
U937(Human) Cell Lysate at 30 ug
Primary: Anti- RNF12 (ER1915-89) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 67 kD
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"