• 概述

• 产品描述 NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases. NLRP3 is a component of the innate immune system that functions as a pattern recognition receptor (PRR) that recognizes pathogen-associated molecular patterns (PAMPs). NLRP3 belongs to the NOD-like receptor (NLR) subfamily of PRRs and NLRP3 together with the adaptor ASC protein PYCARD forms a caspase-1 activating complex known as the NLRP3 inflammasome. NLRP3 in the absence of activating signal is kept in an inactive state complexed with HSP90 and SGT1 in the cytoplasm. NLRP3 inflammasome detects danger signals such as crystalline uric acid and extracellular ATP released by damaged cells. These signals release HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex. Caspase-1 within the activated NLRP3 inflammasome complex in turn activates the inflammatory cytokine, IL-1β. The NLRP3 inflammasome appears to be activated by changes in intracellular potassium caused by potassium efflux from mechanosensitive ion channels located in the cell membrane
• 产品名称 Anti-NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23]
• 分子量 Predicted band size: 118 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 WB,IF-Cell,IF-Tissue,IHC-P,FC,IP
• 抗体类型 重组兔单抗
• 免疫原 Recombinant protein within Human NLRP3 aa 5-161 / 1036.
• 偶联 Non-conjugated

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.
• 数据链接 SwissProt: Q96P20 Human
  SwissProt: Q8R4B8 Mouse
  Entrez Gene: 287362 Rat
  
• 其它名称
  • AGTAVPRL antibody
  • AII/AVP antibody
  • Angiotensin/vasopressin receptor AII/AVP like antibody
  • Angiotensin/vasopressin receptor AII/AVP-like antibody
  • C1orf7 antibody
  • Caterpiller protein 1.1 antibody
  • CIAS 1 antibody
  • CIAS1 antibody
  • CLR1.1 antibody
  • Cold autoinflammatory syndrome 1 antibody
  • Cold autoinflammatory syndrome 1 protein antibody
  • Cryopyrin antibody
  • Familial cold autoinflammatory syndrome antibody
  • FCAS antibody
  • FCU antibody
  • LRR and PYD domains-containing protein 3 antibody
  • Muckle-Wells syndrome antibody
  • MWS antibody
  • NACHT antibody
  • NACHT LRR and PYD containing protein 3 antibody
  • NALP 3 antibody
  • NALP3 antibody
  • NALP3_HUMAN antibody
  • NLRP3 antibody
  • PYPAF 1 antibody
  • PYPAF1 antibody
  • PYRIN containing APAF1 like protein 1 antibody
  • PYRIN-containing APAF1-like protein 1 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IF-Cell: 1:100-1:500
  IF-Tissue: 1:100-1:500
  IHC-P: 1:50-1:400
  FC: 1:50-1:100
  IP: Use at an assay dependent concentration.

• 

  Fig1: Western blot analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: ICC staining of NLRP3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig3: Immunocytochemistry analysis of HUVEC cells labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  

  Fig4: ICC staining of NLRP3 in PMVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig8: Flow cytometric analysis of NLRP3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-93, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  

  Fig9: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/400 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig10: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution.
  
  The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ET1610-93) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  

  Fig11: Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/1,000 dilution.
  
  Lane 1: THP-1 cell lysate
  Lane 2: RAW264.7 cell lysate
  
  Lysates/proteins at 20 µg/Lane.
  
  Predicted band size: 118 kDa
  Observed band size: 118 kDa
  
  Exposure time: 3 minutes;
  
  4-20% SDS-PAGE gel.
  
  Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.

• 背景文献

• 1. Qiao J et al. Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in mice after hematopoietic stem cell transplantation. Sci Rep 5:17828 (2015).
  
  2. Ataide MA et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014).

• Publishing research using Anti-NLRP3 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  37021049 Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling Author: Ding, L., Liao, T., Yang, N., Wei, Y., Xing, R., Wu, P., Li, X., Mao, J., & Wang, P. Published: 2023 Application: WB,IF-Cell Species: Rat Journal: Front Pharmacol IF: 5.988
• PMID:
  36456961 Gastrodin improves neuroinflammation-induced cognitive dysfunction in rats by regulating NLRP3 inflammasome Author: Zheng, X., Gong, T., Tang, C., Zhong, Y., Shi, L., Fang, X., Chen, D., & Zhu, Z. Published: 2022 Application: WB Species: Rat Journal: BMC Anesthesiol. IF: 2.376
• PMID:
  36466156 Targeting NLRP3 Inflammasome Alleviates Synovitis by Reducing Pyroptosis in Rats with Experimental Temporomandibular Joint Osteoarthritis Author: Xin, Y., Wang, W., Mao, E., Yang, H., & Li, S. Published: 2022 Application: IHC,WB Species: Rat Journal: Mediators Inflamm. IF: 4.529
• PMID:
  36076588 Oxytocin ameliorates high glucose- and ischemia/reperfusion-induced myocardial injury by suppressing pyroptosis via AMPK signaling pathway Author: Yao, M., Wang, Z., Jiang, L., Wang, L., Yang, Y., Wang, Q., Qian, X., Zeng, W., Yang, W., Liang, R., & Qian, J. Published: 2022 Application: WB Species: Rat Journal: Biomed Pharmacother. IF: 7.419
• PMID:
  37052047 Lactobacillus plantarum postbiotics trigger AMPK-dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation Author: Wu, Y., Hu, A., Shu, X., Huang, W., Zhang, R., Xu, Y., & Yang, C. Published: 2022 Application: WB Species: Pig Journal: J Cell Physiol . IF: 6.513
• PMID:
  36338340 Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals Author: Jiang, H., Chen, F., Song, D., Zhou, X., Ren, L., & Zeng, M. Published: 2022 Application: WB Species: Mouse Journal: Oxid Med Cell Longev. IF: 7.310
• PMID:
  33549727 Microglial TLR4-induced TAK1 phosphorylation and NLRP3 activation mediates neuroinflammation and contributes to chronic morphine-induced antinociceptive tolerance. Pharmacological research, 165, 105482. Author: Wang, H., Huang, M., Wang, W., Zhang, Y., Ma, X., Luo, L., Xu, X., Xu, L., Shi, H., Xu, Y., Wang, A., & Xu, T. Published: 2021 Application: WB Species: Mouse Journal: Pharmacol Res IF: 5.57
• PMID:
  34912499 Protective Effects of the Soluble Receptor for Advanced Glycation End-Products on Pyroptosis during Myocardial Ischemia-Reperfusion Author: Liu, Y., Guo, X., Zhang, J., Han, X., Wang, H., Du, F., Zeng, X., & Guo, C. Published: 2021 Application: IHC Species: Mouse Journal: Oxid Med Cell Longev IF: 6.543
• PMID:
  33061523 Spinal TLR4/P2X7 Receptor-Dependent NLRP3 Inflammasome Activation Contributes to the Development of Tolerance to Morphine-Induced Antinociception Author: Tao Xu;Aizhong Wang Published: 2020 Application: WB Species: mice Journal: Journal of Inflammation Research IF: 3.174
• PMID:
  33019975 Macrophage pyroptosis is mediated by immunoproteasome subunit β5i (LMP7) in abdominal aortic aneurysm Author: Zhang, X., Li, F., Wang, W., Ji, L., Sun, B., Xiao, X., Wang, X., Chen, Y., Liu, B., Ye, W., Tian, C., Wang, H., & Zheng, Y. Published: 2020 Journal: Biochem Biophys Res Commun. IF: 3.575
• PMID:
  31071330 AT-533,a Hsp90 inhibitor,attenuates HSV-1-induced inflammation. Author: Kai Zheng,Yifei Wang Published: 2019 Application: WB,CO-IP Species: BV2 cells Journal: Biochem Pharmacol. IF: 4.825
• PMID:
  31250434 The effect of NLRP inflammasome on the regulation of AGEs‐ induced inflammatory response in human periodontal ligament cells Author: Dingming Huang ; Lan Zhang Published: 2019 Application: WB Species: Human periodontal ligament cells Journal: J Periodontal Res. IF: 2.613

Choose your product,application and species below,then click "Submit".

On the next page you can complete and submit your review.

Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.