概述
产品名称
NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human NLRP3 aa 5-161 / 1036.
种属反应性
Human, Mouse, Rat
验证应用
WB, IF-Cell, IHC-P, FC
分子量
Predicted band size: 118 kDa
阳性对照
Human lung tissue lysates, Hela, PMVEC, mouse bladder tissue, mouse spleen tissue, human lung carcinoma tissue, Jurkat, THP-1 cell lysate, RAW264.7 cell lysate.
偶联
unconjugated
克隆号
SC06-23
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
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1:500-1:1,000
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IF-Cell
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1:100-1:500
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IHC-P
-
1:50-1:400
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FC
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1:50-1:100
发表文章中的应用
靶点
功能
NLRP3 is expressed predominantly in macrophages and as a component of the inflammasome, detects products of damaged cells such as extracellular ATP and crystalline uric acid. Activated NLRP3 in turn triggers an immune response. Mutations in the NLRP3 gene are associated with a number of organ specific autoimmune diseases. NLRP3 is a component of the innate immune system that functions as a pattern recognition receptor (PRR) that recognizes pathogen-associated molecular patterns (PAMPs). NLRP3 belongs to the NOD-like receptor (NLR) subfamily of PRRs and NLRP3 together with the adaptor ASC protein PYCARD forms a caspase-1 activating complex known as the NLRP3 inflammasome. NLRP3 in the absence of activating signal is kept in an inactive state complexed with HSP90 and SGT1 in the cytoplasm. NLRP3 inflammasome detects danger signals such as crystalline uric acid and extracellular ATP released by damaged cells. These signals release HSP90 and SGT1 from and recruit ASC protein and caspase-1 to the inflammasome complex. Caspase-1 within the activated NLRP3 inflammasome complex in turn activates the inflammatory cytokine, IL-1β. The NLRP3 inflammasome appears to be activated by changes in intracellular potassium caused by potassium efflux from mechanosensitive ion channels located in the cell membrane
背景文献
1. Qiao J et al. Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in mice after hematopoietic stem cell transplantation. Sci Rep 5:17828 (2015).
2. Ataide MA et al. Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection. PLoS Pathog 10:e1003885 (2014).
序列相似性
Belongs to the NLRP family.
组织特异性
Predominantly expressed in macrophages. Also expressed in dendritic cells, B- and T-cells (at protein level). Expressed in LPS-treated granulocytes, but not in resting cells (at protein level). Expression in monocytes is very weak (at protein level). Expressed in stratified non-keratinizing squamous epithelium, including oral, esophageal and ectocervical mucosa and in the Hassall's corpuscles in the thymus. Also, detected in the stratified epithelium covering the bladder and ureter (transitional mucosa) (at protein level). Expressed in lung epithelial cells (at protein level). Expressed in chondrocytes. Expressed at low levels in resting osteoblasts.
翻译后修饰
The disulfide bond in the pyrin domain might play a role in reactive oxygen species-mediated activation.; Ubiquitinated; undergoes both 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Ubiquitination does not lead to degradation, but inhibits inflammasome activation (By similarity). Deubiquitination is catalyzed by BRCC3 and associated with NLRP3 activation and inflammasome assembly. This process can be induced by the activation of Toll-like receptors (by LPS), through a non-transcriptional pathway dependent on the mitochondrial production of reactive oxygen species, and by ATP.
亚细胞定位
Cytoplasm, Inflammasome, Secreted, Nucleus, Endoplasmic reticulum.
UNIPROT #
别名
AGTAVPRL antibody
AII/AVP antibody
Angiotensin/vasopressin receptor AII/AVP like antibody
Angiotensin/vasopressin receptor AII/AVP-like antibody
C1orf7 antibody
Caterpiller protein 1.1 antibody
CIAS 1 antibody
CIAS1 antibody
CLR1.1 antibody
Cold autoinflammatory syndrome 1 antibody
展开AGTAVPRL antibody
AII/AVP antibody
Angiotensin/vasopressin receptor AII/AVP like antibody
Angiotensin/vasopressin receptor AII/AVP-like antibody
C1orf7 antibody
Caterpiller protein 1.1 antibody
CIAS 1 antibody
CIAS1 antibody
CLR1.1 antibody
Cold autoinflammatory syndrome 1 antibody
Cold autoinflammatory syndrome 1 protein antibody
Cryopyrin antibody
Familial cold autoinflammatory syndrome antibody
FCAS antibody
FCU antibody
LRR and PYD domains-containing protein 3 antibody
Muckle-Wells syndrome antibody
MWS antibody
NACHT antibody
NACHT LRR and PYD containing protein 3 antibody
NALP 3 antibody
NALP3 antibody
NALP3_HUMAN antibody
NLRP3 antibody
PYPAF 1 antibody
PYPAF1 antibody
PYRIN containing APAF1 like protein 1 antibody
PYRIN-containing APAF1-like protein 1 antibody
折叠图片
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Western blot analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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ICC staining of NLRP3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunocytochemistry analysis of HUVEC cells labeling NLRP3 with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. -
ICC staining of NLRP3 in PMVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1610-93, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Flow cytometric analysis of NLRP3 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1610-93, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1610-93) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of NLRP3 on different lysates with Rabbit anti-NLRP3 antibody (ET1610-93) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: RAW264.7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 118 kDa
Observed band size: 118 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-93) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3
Author: Liu Li,et al
PMID: 38158667
应用: WB
反应种属: Rat
发表时间: 2024 Jan
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Citation
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Fucoidan modulates SIRT1 and NLRP3 to alleviate hypertensive retinopathy: in vivo and in vitro insights
Author: Li Jing,et al
PMID: 38360728
应用: IHC,WB
反应种属: Mouse
发表时间: 2024 Feb
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Citation
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Polydatin improves vascular endothelial function by maintaining mitochondrial homeostasis under high glucose conditions
Author: Shah W, Zhao Q, Wang S, et al
PMID: 37783713
应用: WB
反应种属: Human
发表时间: 2023 Oct
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Citation
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Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice
Author:
PMID: 37893938
应用: WB
反应种属: Mouse
发表时间: 2023 Oct
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Citation
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Supramolecular salicylic acid ameliorates rosacea-like eruptions by suppressing NLRP3-mediated inflammasome activation in mice
Author:
PMID: 36989903
应用: IHC
反应种属: Mouse
发表时间: 2023 May
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Citation
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PD184352 exerts anti-inflammatory and antioxidant effects by promoting activation of the Nrf2/HO-1 axis
Author:
PMID: 37028460
应用: IF
反应种属: Mouse
发表时间: 2023 May
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Citation
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Functionalized Metal-Organic Framework-Modified Hydrogel That Breaks the Vicious Cycle of Inflammation and ROS for Repairing of Diabetic Bone Defects
Author: Lao, A., Wu, J., Li, D., Shen, A., Li, Y., Zhuang, Y., Lin, K., Wu, J., & Liu, J.
PMID: 37183293
应用: WB,IF
反应种属: Rat
发表时间: 2023 May
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Citation
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CVB3 Inhibits NLRP3 Inflammasome Activation by Suppressing NF-κB Pathway and ROS Production in LPS-Induced Macrophages
Author: Wang, Y., Sun, Z., Zhang, H., Song, Y., Wang, Y., Xu, W., & Li, M.
PMID: 37243164
应用: WB
反应种属: Mouse
发表时间: 2023 May
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Citation
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Irisin attenuates fine particulate matter induced acute lung injury by regulating Nod2/NF-κB signaling pathway
Author:
PMID: 37003140
应用: ICC
反应种属: Mouse
发表时间: 2023 May
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Citation
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Small molecule inhibitor CRT0066101 inhibits cytokine storm syndrome in a mouse model of lung injury
Author: Cui, B., Liu, Y., Chen, J., Chen, H., Feng, Y., & Zhang, P.
PMID: 37182445
应用: WB,IF
反应种属: Mouse
发表时间: 2023 May
-
Citation
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IL‐1β promotes IL‐17A production of ILC3s to aggravate neutrophilic airway inflammation in mice
Author:
PMID: 36988516
应用: IHC
反应种属: Mouse
发表时间: 2023 Mar
-
Citation
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Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signaling
Author: Ding, L., Liao, T., Yang, N., Wei, Y., Xing, R., Wu, P., Li, X., Mao, J., & Wang, P.
PMID: 37021049
应用: WB,IF-Cell
反应种属: Rat
发表时间: 2023 Mar
-
Citation
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Sevoflurane preconditioning improves neuroinflammation in cerebral ischemia/reperfusion induced rats through ROS-NLRP3 pathway
Author:
PMID: 36868396
应用: WB
反应种属: Rat
发表时间: 2023 Mar
-
Citation
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Formononetin alleviates acute pancreatitis by reducing oxidative stress and modulating intestinal barrier
Author:
PMID: 37370098
应用: WB
反应种属: Mouse
发表时间: 2023 Jun
-
Citation
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Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion——From the perspective of glial-vascular unit
Author:
PMID: 37536647
应用: WB
反应种属: Rat
发表时间: 2023 Aug
-
Citation
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NLRP10 promotes AGEs-induced NLRP1 and NLRP3 inflammasome activation via ROS/MAPK/NF-κB signaling in human periodontal ligament cells
Author: Yi X, Song Y, Xu J, et al
PMID: 37043073
应用: WB
反应种属: Human
发表时间: 2023 Apr
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Citation
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Oral Delivery of Mouse β-Defensin 14 (mBD14)-Producing Lactococcus lactis NZ9000 Attenuates Experimental Colitis in Mice
Author:
PMID: 36943701
应用: WB
反应种属: Mouse
发表时间: 2023 Apr
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Citation
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Contribution of diabetes mellitus to periodontal inflammation during orthodontic tooth movement
Author:
PMID: 36050281
应用: WB, IHC
反应种属: Rat
发表时间: 2022 Sep
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Citation
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Dehydromevalonolactone ameliorates liver fibrosis and inflammation by repressing activation of NLRP3 inflammasome
Author:
PMID: 35749855
应用: WB
反应种属: Human
发表时间: 2022 Oct
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Citation
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Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals
Author: Jiang, H., Chen, F., Song, D., Zhou, X., Ren, L., & Zeng, M.
PMID: 36338340
应用: WB
反应种属: Mouse
发表时间: 2022 Oct
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Citation
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Targeting NLRP3 Inflammasome Alleviates Synovitis by Reducing Pyroptosis in Rats with Experimental Temporomandibular Joint Osteoarthritis
Author: Xin, Y., Wang, W., Mao, E., Yang, H., & Li, S.
PMID: 36466156
应用: IHC,WB
反应种属: Rat
发表时间: 2022 Nov
-
Citation
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Voluven treatment could induce NLRP3 inflammasome-mediated pyroptosis of bone marrow-derived macrophages
Author:
PMID: 35413207
应用: WB
反应种属: Mouse
发表时间: 2022 Jul
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Citation
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Ginsenoside Rh2 Inhibits NLRP3 Inflammasome Activation and Improves Exosomes to Alleviate Hypoxia-Induced Myocardial Injury
Author:
PMID: 35865525
应用: ICC
反应种属: Rat
发表时间: 2022 Jul
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Citation
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Ginsenoside Rh2 Inhibits NLRP3 Inflammasome Activation and Improves Exosomes to Alleviate Hypoxia-Induced Myocardial Injury
Author:
PMID: 35865525
应用: WB
反应种属: Rat
发表时间: 2022 Jul
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Citation
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Gastrodin improves neuroinflammation-induced cognitive dysfunction in rats by regulating NLRP3 inflammasome
Author: Zheng, X., Gong, T., Tang, C., Zhong, Y., Shi, L., Fang, X., Chen, D., & Zhu, Z.
PMID: 36456961
应用: WB
反应种属: Rat
发表时间: 2022 Dec
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Citation
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Oxytocin ameliorates high glucose- and ischemia/reperfusion-induced myocardial injury by suppressing pyroptosis via AMPK signaling pathway
Author: Yao, M., Wang, Z., Jiang, L., Wang, L., Yang, Y., Wang, Q., Qian, X., Zeng, W., Yang, W., Liang, R., & Qian, J.
PMID: 36076588
应用: WB
反应种属: Rat
发表时间: 2022 Aug
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Citation
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Lactobacillus plantarum postbiotics trigger AMPK-dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation
Author: Wu, Y., Hu, A., Shu, X., Huang, W., Zhang, R., Xu, Y., & Yang, C.
PMID: 37052047
应用: WB
反应种属: Pig
发表时间: 2022 Apr
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Citation
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Microglial TLR4-induced TAK1 phosphorylation and NLRP3 activation mediates neuroinflammation and contributes to chronic morphine-induced antinociceptive tolerance
Author: Wang, H., Huang, M., Wang, W., Zhang, Y., Ma, X., Luo, L., Xu, X., Xu, L., Shi, H., Xu, Y., Wang, A., & Xu, T.
PMID: 33549727
应用: WB
反应种属: Mouse
发表时间: 2021 Mar
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Citation
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Protective Effects of the Soluble Receptor for Advanced Glycation End-Products on Pyroptosis during Myocardial Ischemia-Reperfusion
Author: Liu, Y., Guo, X., Zhang, J., Han, X., Wang, H., Du, F., Zeng, X., & Guo, C.
PMID: 34912499
应用: IHC
反应种属: Mouse
发表时间: 2021 Dec
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Citation
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Spinal TLR4/P2X7 Receptor-Dependent NLRP3 Inflammasome Activation Contributes to the Development of Tolerance to Morphine-Induced Antinociception
Author: Tao Xu;Aizhong Wang
PMID: 33061523
应用: WB
反应种属: mice
发表时间: 2020 Sep
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Citation
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Inhibition of P2X7 Purinergic Receptor Ameliorates Cardiac Fibrosis by Suppressing NLRP3/IL-1β Pathway
Author:
PMID: 32566102
应用: WB
反应种属: Mouse
发表时间: 2020 May
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Citation
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Dendrobium officinale polysaccharides protected against ethanol-induced acute liver injury in vivo and in vitro via the TLR4/NF-κB signaling pathway
Author:
PMID: 32222695
应用: WB
反应种属: Rat
发表时间: 2020 Mar
-
Citation
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HSP60 Regulates Monosodium Urate Crystal-Induced Inflammation by Activating the TLR4-NF-κB-MyD88 Signaling Pathway and Disrupting Mitochondrial Function
Author:
PMID: 33488933
应用: WB
反应种属: Human
发表时间: 2020 Dec
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Citation
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Macrophage pyroptosis is mediated by immunoproteasome subunit β5i (LMP7) in abdominal aortic aneurysm
Author: Zhang, X., Li, F., Wang, W., Ji, L., Sun, B., Xiao, X., Wang, X., Chen, Y., Liu, B., Ye, W., Tian, C., Wang, H., & Zheng, Y.
PMID: 33019975
应用:
反应种属:
发表时间: 2020 Dec
-
Citation
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The effect of NLRP inflammasome on the regulation of AGEs‐ induced inflammatory response in human periodontal ligament cells
Author: Dingming Huang ; Lan Zhang
PMID: 31250434
应用: WB
反应种属: Human periodontal ligament cells
发表时间: 2019 Dec
-
Citation
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Curcumin attenuates MSU crystal-induced inflammation by inhibiting the degradation of IκBα and blocking mitochondrial damage.
Author:
PMID: 31455356
应用: WB
反应种属: Mouse
发表时间: 2019 Aug
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Citation
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AT-533,a Hsp90 inhibitor,attenuates HSV-1-induced inflammation.
Author: Kai Zheng,Yifei Wang
PMID: 31071330
应用: WB,CO-IP
反应种属: BV2 cells
发表时间: 2019 Aug
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Citation
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Dietary Zinc Ameliorates TNBS-Induced Colitis in Mice Associated with Regulation of Th1/Th2/Th17 Balance and NF-κB/NLRP3 Signaling Pathway.
Author:
PMID: 37249802
应用: WB
反应种属: Mouse
发表时间:
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Citation