Mature Neuron Marker Antibody Kit
概述
试剂盒组分
产品包括 | 规格 | 应用 | 反应性 | MW(kDa) |
---|---|---|---|---|
NeuN[ET1602-12] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC,IHC-Fr | Human,Mouse,Rat | Predicted band size: 34 kDa |
GAP43[ET1610-94] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC,IP | Human,Mouse,Rat | Predicted band size: 25 kDa |
MAP2[HA500177] | 20µl | WB,IF-Cell,IHC-P | Human,Mouse,Rat | 200/59/50 kDa |
NF-L[ER65439] | 20µl | WB,IHC-P | Human,Mouse,Rat | 61 kDa |
Beta III Tubulin[ET1604-17] | 20µl | WB,IHC-P,IP,FC,IHC-Fr | Human,Mouse,Rat | Predicted band size: 50 kDa |
Synaptophysin[ET1606-56] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P | Human,Mouse,Rat | Predicted band size: 34 kDa |
PSD95[ET1602-20] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,FC,IP | Human,Mouse,Rat | Predicted band size: 80 kDa |
PGP9.5[ET1703-22] | 20µl | WB,IF-Cell,IF-Tissue,IHC-P,IP | Human,Mouse,Rat | Predicted band size: 25 kDa |
HRP Conjugated Alpaca anti-Rabbit IgG FC, Recombinant VHH[HA1031] | 100µl | IP,ELISA,IHC-P,WB | Rabbit |
产品描述
Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. NeuN is not detected in Purkinje cells, sympathetic ganglion cells, Cajal-Retzius cells, INL retinal cells, inferior olivary, and dentate nucleus neurons. This neuronal protein was originally identified by immunoreactivity with a monoclonal antibody also called NeuN. Using MS-analysis, NeuN was later identified as the Fox-3 gene product. Fox-3 contains an RNA recognition motif and functions as a splicing regulator. Fox-3 regulates alternative splicing of NumB, promoting neuronal differentiation during development.
产品特性
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
背景
The antibodies in this kit serve to characterize and identify mature neurons. Neuronal nuclei (NeuN, Fox-3, RBFOX3) is a nuclear protein expressed in most post-mitotic neurons of the central and peripheral nervous systems. </br>GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity. β3-tubulin is one of six β-tubulin isoforms that make up the building blocks of microtubules. Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits. Synaptophysin is a neuronal synaptic vesicle glycoprotein. Synaptophysin is responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, and is a critical component and marker for the presynaptic fusion complex. PSD95 is a scaffolding protein involved in the assembly and function of mature postsynaptic density complexes.
数据链接
SwissProt: Q8BIF2 Mouse
Unigene: 143966 Rat
SwissProt: P17677 Human
SwissProt: P06837 Mouse
SwissProt: P07936 Rat
SwissProt: P11137 Human
SwissProt: P20357 Mouse
SwissProt: P15146 Rat
SwissProt: P07196 Human
SwissProt: Q13509 Human
SwissProt: Q9ERD7 Mouse
SwissProt: Q4QRB4 Rat
SwissProt: P08247 Human
SwissProt: Q62277 Mouse
SwissProt: P07825 Rat
SwissProt: P78352 Human
SwissProt: Q62108 Mouse
SwissProt: P31016 Rat
SwissProt: P09936 Human
SwissProt: Q9R0P9 Mouse
SwissProt: Q00981 Rat
SwissProt: P42574 Human
背景文献
2. Kim, K.K. et al. (2009) J Biol Chem 284, 31052-61.
3. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
5. Jiang, Y.Q. and Oblinger, M.M. (1992) J Cell Sci 103 (Pt 3), 643-51.
6. Sánchez, C. et al. (2000) Prog Neurobiol 61, 133-68.
7. Caceres, A. et al. (1984) Brain Res 315, 314-8.
8. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-55.
9. Cohlberg, J.A. et al. (1995) J Biol Chem 270, 9334-9.
10. Wiedenmann, B. and Franke, W.W. (1985) Cell 41, 1017-28.
11. Bonanomi, D. et al. (2007) Biochem J 404, 525-34.
12. Cao, J. et al. (2005) J Cell Biol 168, 117-26.
13. Chetkovich, D.M. et al. (2002) J Neurosci 22, 6415-25.
14. Gong, B. et al. (2006) Cell 126, 775-88.
图片
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Western blot analysis of NeuN on different lysates with Rabbit anti-NeuN antibody (ET1602-12) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: Rat cerebellum tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 45/50 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-12) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: Neuro-2a cell lysate
Lane 3: Mouse brain tissue lysate
Lane 4: Rat brain tissue lysate
Lane 5: Rat hippocampus tissue lysate
Lane 6: Mouse hippocampus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 43/45 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of GAP43 on different lysates with Rabbit anti-GAP43 antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: A549 cell lysate (negative)
Lane 3: Neuro-2a cell lysate
Lane 4: Mouse lung tissue lysate (negative)
Lane 5: Mouse brain tissue lysate
Lane 6: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 43/45 kDa
Exposure time: 3 minutes 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-94) at 1/100,000 dilution and competitor's antibody at 1/100,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Beta III Tubulin on different lysates with Rabbit anti-Beta III Tubulin antibody (ET1604-17) at 1/20,000 dilution.
Lane 1: SH-SY5Y cell lysate (15 µg/Lane)
Lane 2: HeLa cell lysate (15 µg/Lane)
Lane 3: Neuro-2a cell lysate (15 µg/Lane)
Lane 4: NIH/3T3 cell lysate (15 µg/Lane)
Lane 5: PC-12 cell lysate (15 µg/Lane)
Lane 6: C6 cell lysate (15 µg/Lane)
Lane 7: Mouse brain tissue lysate (20 µg/Lane)
Lane 8: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 3 minutes 54 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1604-17) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Synaptophysin on different lysates with Rabbit anti-Synaptophysin antibody (ET1606-56) at 1/5,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: HeLa cell lysate (negative)
Lane 3: NIH/3T3 cell lysate (negative)
Lane 4: PC-12 cell lysate
Lane 5: Human brain tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34/40 kDa
Exposure time: 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-56) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PSD95 on different lysates with Rabbit anti-PSD95 antibody (ET1602-20) at 1/2,000 dilution.
Lane 1: C6 cell lysate (15 µg/Lane)
Lane 2: Mouse brain tissue lysate (20 µg/Lane)
Lane 3: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 80 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes 10 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-20) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.