• 概述

• 产品描述 This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.
• 产品名称 iFluor™ 647 Conjugated Anti-MUC1 Mouse Monoclonal Antibody [1A9-5-6]
• 分子量 Predicted band size: 122 kDa
• 种属反应性 Human
• 验证应用 FC
• 抗体类型 小鼠单抗
• 免疫原 Synthetic peptide of core peptide domain of human MUC1.
• 偶联 iFluor™ 647

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG2a
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Apical cell membrane. Secreted. Nucleus, Cell membrane, Cytoplasm.
• 数据链接 SwissProt: P15941 Human
  
• 其它名称
  • ADMCKD antibody
  • ADMCKD1 antibody
  • Breast carcinoma associated antigen DF3 antibody
  • Breast carcinoma-associated antigen DF3 antibody
  • CA 15-3 antibody
  • CA15 3 antibody
  • CA15 3 antigen antibody
  • CA15-3 antibody
  • CA15.3 antibody
  • Cancer antigen 15-3 antibody
  • Carcinoma associated mucin antibody
  • Carcinoma-associated mucin antibody
  • CD 227 antibody
  • CD227 antibody
  • DF3 antigen antibody
  • EMA antibody
  • Episialin antibody
  • Epithelial Membrane Antigen antibody
  • H23 antigen antibody
  • H23AG antibody
  • KL 6 antibody
  • KL-6 antibody
  • KL6 antibody
  • Krebs von den Lungen-6 antibody
  • MAM 6 antibody
  • MAM6 antibody
  • MCD antibody
  • MCKD antibody
  • MCKD1 antibody
  • Medullary cystic kidney disease 1 (autosomal dominant) antibody
  • Medullary cystic kidney disease, autosomal dominant antibody
  • MUC 1 antibody
  • MUC-1 antibody
  • MUC-1/SEC antibody
  • MUC-1/X antibody
  • MUC1 antibody
  • MUC1-alpha antibody
  • MUC1-beta antibody
  • MUC1-CT antibody
  • MUC1-NT antibody
  • MUC1/ZD antibody
  • MUC1_HUMAN antibody
  • Mucin 1 antibody
  • Mucin 1 cell surface associated antibody
  • Mucin 1 transmembrane antibody
  • Mucin 1, cell surface associated antibody
  • Mucin-1 subunit beta antibody
  • Peanut reactive urinary mucin antibody
  • Peanut-reactive urinary mucin antibody
  • PEM antibody
  • PEMT antibody
  • Polymorphic epithelial mucin antibody
  • PUM antibody
  • Tumor associated epithelial membrane antigen antibody
  • Tumor associated epithelial mucin antibody
  • Tumor associated mucin antibody
  • Tumor-associated epithelial membrane antigen antibody
  • Tumor-associated mucin antibody
  • iFluor™647ConjugatedAnti-MUC1
  • iFluor™647ConjugatedAntiMUC1
  more

• 应用

  FC: 1:500-1:1,000

• 

  Fig1: Flow cytometric analysis of MCF7 cells labeling MUC1.
  
  Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with MUC1 (HA600107F, red, 1ug/ml) and Mouse IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  

  Fig2: Flow cytometric analysis of SK-Br-3 cells labeling MUC1.
  
  Cells were washed twice with cold PBS and resuspend. Then incubated for 1 hour at +4℃ with MUC1 (HA600107F, red, 1ug/ml) and Mouse IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. Taylor-Papadimitriou J. et. al. Latest developments in MUC1 immunotherapy. Biochem Soc Trans. 2018 Jun
  
  2. Kato K. et. al. MUC1: The First Respiratory Mucin with an Anti-Inflammatory Function. J Clin Med. 2017 Nov

• Publishing research using iFluor™ 647 Conjugated Anti-MUC1 Mouse Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.