• 概述

• 产品描述 Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter, Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane, and shows a much less dramatic translocation in response to insulin. Glut1 and Glut4 are twelve-pass transmembrane proteins (12TM) whose carboxy-termini may dictate their cellular localization. Aberrant Glut4 expression has been suggested to contribute to such maladies as obesity and diabetes. Glut4 null mice have shown that while functional Glut4 protein is not required for maintaining normal glucose levels, it is necessary for sustained growth, normal cellular glucose, fat metabolism and prolonged longevity.
• 产品名称 iFluor™ 488 Conjugated Anti-Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377]
• 分子量 Predicted band size: 54 kDa
• 种属反应性 Human,Mouse, Rat
• 验证应用 IF-Cell,IF-Tissue
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human GLUT1 aa 443-492 / 492.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cell membrane, Melanosome
• 数据链接 SwissProt: P11166 Human
  SwissProt: P17809 Mouse
  SwissProt: P11167 Rat
  
• 其它名称
  • Choreoathetosis/spasticity episodic (paroxysmal choreoathetosis/spasticity) antibody
  • CSE antibody
  • DYT17 antibody
  • DYT18 antibody
  • DYT9 antibody
  • EIG12 antibody
  • erythrocyte/brain antibody
  • Erythrocyte/hepatoma glucose transporter antibody
  • facilitated glucose transporter member 1 antibody
  • Glucose transporter 1 antibody
  • Glucose transporter type 1 antibody
  • Glucose transporter type 1, erythrocyte/brain antibody
  • GLUT antibody
  • GLUT-1 antibody
  • GLUT1 antibody
  • GLUT1DS antibody
  • GLUTB antibody
  • GT1 antibody
  • GTG1 antibody
  • Gtg3 antibody
  • GTR1_HUMAN antibody
  • HepG2 glucose transporter antibody
  • HTLVR antibody
  • Human T cell leukemia virus (I and II) receptor antibody
  • MGC141895 antibody
  • MGC141896 antibody
  • PED antibody
  • RATGTG1 antibody
  • Receptor for HTLV 1 and HTLV 2 antibody
  • SLC2A1 antibody
  • Solute carrier family 2 (facilitated glucose transporter), member 1 antibody
  • Solute carrier family 2 antibody
  • Solute carrier family 2, facilitated glucose transporter member 1 antibody
  • iFluor™488ConjugatedAnti-GlucoseTransporterGLUT1
  • iFluor™488ConjugatedAntiGlucoseTransporterGLUT1
  more

• 应用

  IF-Cell: 1:100
  IF-Tissue: 1:50

• 

  Fig1: Immunocytochemistry analysis of MCF7 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  

  Fig2: Immunofluorescence analysis of paraffin-embedded human placenta tissue labeling Glucose Transporter GLUT1 (HA720154F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Glucose Transporter GLUT1 (HA720154F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  

  Fig3: Immunocytochemistry analysis of MDA-MB-468 cells labeling Glucose Transporter GLUT1 with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Glucose Transporter GLUT1 antibody (HA720154F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

• 背景文献

• 1. Boyer-Di Ponio J et al. Instruction of circulating endothelial progenitors in vitro towards specialized blood-brain barrier and arterial phenotypes. PLoS One 9:e84179 (2014).
  
  2. Saucillo DC et al. Leptin metabolically licenses T cells for activation to link nutrition and immunity. J Immunol 192:136-44 (2014).

• Publishing research using iFluor™ 488 Conjugated Anti-Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.