概述
产品名称
GFP Recombinant Rabbit Monoclonal Antibody [SY0243]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Synthetic peptide within Aequorea victoria GFP aa 1-50 / 238.
种属反应性
Species independent
验证应用
WB, IF-Cell, IF-Tissue, IP
分子量
27 kD
阳性对照
GFP recombinant protein.
偶联
unconjugated
克隆号
SY0243
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
-
WB
-
1:10,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IP
-
2-5 µg/ml.
发表文章中的应用
靶点
功能
The green fluorescent protein (GFP) was originally identified as a protein involved in the bioluminescence of the jellyfish Aequorea victoria. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors, making GFP a useful tool for monitoring gene expression and protein localization in vivo. Several GFP mutants have been developed, including EGFP, which fluoresce more intensely than the wildtype GFP and have shifted excitation maxima, making them useful for FACS and fluorescence microscopy as well as double-labeling applications. GFP is widely used in expression vectors as a fusion protein tag, allowing expression and monitoring of heterologous proteins fused to GFP.
背景文献
1. Yu, H. et al. 2016. AAV-Mediated Gene Transfer to Dorsal Root Ganglion. Methods in molecular biology (Clifton, N.J.). 1382: 251-61.
2. Yamaoka, M. et al. 2016. PI3K regulates endocytosis after insulin secretion by mediating signaling crosstalk between Arf6 and Rab27a. J. Cell. Sci. 129: 637-49.
UNIPROT #
别名
GFP antibody
Green fluorescent protein antibody
yfp antibody
图片
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Western blot analysis of GFP on GFP recombinant protein with Rabbit anti-GFP antibody (ET1607-31) at 1/10,000 dilution.
Lysates/proteins at 50 ng/Lane.
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-31) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature. -
GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with ET1607-31 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: Rabbit IgG instead of ET1607-31 in GFP Tag fusion protein lysate.
Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST -
Immunocytochemistry analysis of HeLa cells transfected with N-terminal GFP labeling GFP with Rabbit anti-GFP antibody (ET1607-31) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GFP antibody (ET1607-31) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with GFP (green). Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
引文
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Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
Author: Deng T, Du L, Ding S, et al
PMID: 37902398
应用: WB
反应种属: Human
发表时间: 2023 Oct
-
Citation
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LASS2 enhances p53 protein stability and nuclear import to suppress liver cancer progression through interaction with MDM2/MDMX
Author: Zhao Q, He W, Liu Z, et al
PMID: 37963859
应用: WB
反应种属: Human
发表时间: 2023 Nov
-
Citation
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MoCbp7, a Novel Calcineurin B Subunit-Binding Protein, Is Involved in the Calcium Signaling Pathway and Regulates Fungal Development, Virulence, and ER Homeostasis in Magnaporthe oryzae
Author:
PMID: 37298247
应用: WB
反应种属:
发表时间: 2023 May
-
Citation
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Heme Oxygenase-1 and Its Metabolites Carbon Monoxide and Biliverdin, but Not Iron, Exert Antiviral Activity against Porcine Circovirus Type 3
Author:
PMID: 37140466
应用: WB
反应种属: Pig
发表时间: 2023 Jun
-
Citation
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A novel transcriptional repressor complex MYB22–TOPLESS–HDAC1 promotes rice resistance to brown planthopper by repressing F3′H expression
Author:
PMID: 37149887
应用: Co-IP
反应种属: Rice
发表时间: 2023 Jul
-
Citation
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DDX5 inhibits type I IFN production by promoting degradation of TBK1 and disrupting formation of TBK1− TRAF3 complex
Author:
PMID: 37462751
应用: WB,IP
反应种属: Human
发表时间: 2023 Jul
-
Citation
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Avian Metapneumovirus Subgroup C Phosphoprotein Suppresses Type I Interferon Production by Blocking Interferon Regulatory Factor 3 Nuclear Translocation
Author:
PMID: 36537793
应用: WB,IF-cell
反应种属: human
发表时间: 2023 Feb
-
Citation
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VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling
Author: Gao XK, Sheng ZK, Lu YH, et al
PMID: 37528084
应用: WB
反应种属: Mouse
发表时间: 2023 Aug
-
Citation
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MoWhi2 Mediates Mitophagy to Regulate Conidiation and Pathogenesis in Magnaporthe oryzae
Author: Meng, S., Jagernath, J. S., Luo, C., Shi, H., & Kou, Y.
PMID: 35628129
应用: WB
反应种属: Magnaporthe oryzae
发表时间: 2022 May
-
Citation
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Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes
Author: Gao, X. K., Rao, X. S., Cong, X. X., Sheng, Z. K., Sun, Y. T., Xu, S. B., Wang, J. F., Liang, Y. H., Lu, L. R., Ouyang, H., Ge, H., Guo, J. S., Wu, H. J., Sun, Q. M., Wu, H. B., Bao, Z., Zheng, L. L., & Zhou, Y. T.
PMID: 35764611
应用: WB
反应种属: Mouse
发表时间: 2022 Jun
-
Citation
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Casein Kinase 2 Mediates Degradation of Transcription Factor Pcf1 during Appressorium Formation in the Rice Blast Fungus
Author: Huang, P., Li, Y., Wang, J., Wang, Q., Huang, Z., Liu, X., Lin, F., & Lu, J.
PMID: 35205898
应用: IP
反应种属: M.oryzae
发表时间: 2022 Jan
-
Citation
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A Subunit of the COP9 Signalosome, MoCsn6, Is Involved in Fungal Development, Pathogenicity, and Autophagy in Rice Blast Fungus
Author:
PMID: 36445131
应用: WB
反应种属: M. Oryzae
发表时间: 2022 Dec
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Citation
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Seneca Valley Virus Enters PK-15 Cells via Caveolae-Mediated Endocytosis and Macropinocytosis Dependent on Low-pH, Dynamin, Rab5, and Rab7
Author: Hou, L., Tong, X., Pan, Y., Shi, R., Liu, C., Guo, J., Shi, Y., Yang, X., Wang, Y., Feng, X., Zhou, J., & Liu, J.
PMID: 36472440
应用:
反应种属:
发表时间: 2022 Dec
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Citation
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The Plant Homeodomain Protein Clp1 Regulates Fungal Development, Virulence, and Autophagy Homeostasis in Magnaporthe oryzae
Author: Wang, J., Huang, Z., Huang, P., Wang, Q., Li, Y., Liu, X. H., Lin, F. C., & Lu, J.
PMID: 36036638
应用: WB,Co-IP
反应种属: Magnaporthe oryzae
发表时间: 2022 Aug
-
Citation
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A Subunit of ESCRT-III, MoIst1, Is Involved in Fungal Development, Pathogenicity, and Autophagy in Magnaporthe oryzae
Author:
PMID: 35463448
应用: WB
反应种属: Pyricularia Oryzae
发表时间: 2022 Apr
-
Citation
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SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
Author: Zhou, D., Yan, H., Yang, S., Zhang, Y., Xu, X., Cen, X., Lei, K., & Xia, H.
PMID: 34776962
应用: WB
反应种属: Human
发表时间: 2021 Oct
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Citation
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<html>Genome-Wide Identification Reveals That <i>Nicotiana benthamiana</i> Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.</html>
Author: Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
PMID: 34113359
应用: WB
反应种属: Nicotiana tabacum
发表时间: 2021 May
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Citation
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A Novel Nitrogen and Carbon Metabolism Regulatory Cascade Is Implicated in Entomopathogenicity of the Fungus Metarhizium robertsii
Author:
PMID: 34156296
应用: WB
反应种属: Yeast
发表时间: 2021 Jun
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Citation
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<html>Inhibitory Role of an <i>Aeromonas hydrophila</i> TIR Domain Effector in Antibacterial Immunity by Targeting TLR Signaling Complexes in Zebrafish. Frontiers in microbiology, 12, 694081.</html>
Author: Tang, H. P., Huang, C., Hu, C. B., Li, H., Shao, T., Ji, J. F., Bai, J., Fan, D. D., Lin, A. F., Xiang, L. X., & Shao, J. Z.
PMID: 34305858
应用: Co-IP
反应种属: Zebrafish
发表时间: 2021 Jul
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Citation
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Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus
Author:
PMID: 33794278
应用: Co-IP
反应种属: Human
发表时间: 2021 Aug
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Citation
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Integrative omic analysis reveals the improvement of alkaloid accumulation by ultraviolet-B radiation and its upstream regulation in Catharanthus roseus
Author:
PMID: 33794278
应用: WB
反应种属: Human
发表时间: 2021 Aug
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Citation
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MoWhi2 regulates appressorium formation and pathogenicity via the MoTor signalling pathway in Magnaporthe oryzae
Author:
PMID: 34036714
应用: Co-IP
反应种属: Rice
发表时间: 2021 Aug
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Citation
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LASS2 regulates hepatocyte steatosis by interacting with NDUFS2/ OXPHOS related proteins
Author:
PMID: 32279995
应用: co-IP
反应种属:
发表时间: 2020 Jun
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Citation
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MoSec61β, the beta subunit of Sec61, is involved in fungal development and pathogenicity, plant immunity, and ER-phagy in Magnaporthe oryzae
Author:
PMID: 33200669
应用: WB
反应种属: Magnaporthe oryzae
发表时间: 2020 Dec
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Citation
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Scophthalmus maximus interleukin-1β limits Edwardsiella piscicida colonization in vivo.
Author:
PMID: 31669781
应用: IF-cell
反应种属: human
发表时间: 2019 Dec
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Citation
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Suppression of RNA Silencing by a Plant DNA Virus Satellite Requires a Host Calmodulin-Like Protein to Repress RDR6 Expression
Author:
PMID: 24516387
应用: WB
反应种属: Virens
发表时间: 2014 Feb
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Citation