• 概述

• 产品描述 The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
• 产品名称 Anti-DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
• 验证应用 WB,IP
• 抗体类型 兔多抗
• 免疫原 Synthetic peptide immune sequence is CDYKDDDDK.
• 偶联 Non-conjugated
• RRID AB_3068715

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型 IgG
• 纯化方式 Immunogen affinity purified.
• 其它名称
  • DDDDK epitope tag antibody
  • DDDK antibody
  • ddk antibody
  • DYKDDDDK antibody
  • DYKDDDDK epitope tag antibody
  • DYKDDDDK tag antibody
  • ECS epitope tag antibody
  • ECS tag antibody
  • Enterokinase Cleavage Site epitope tag antibody
  • Enterokinase Cleavage Site tag antibody
  • FLAG antibody
  • FLAG tag antibody
  • DYKDDDDKTag(FLAG)
  more

• 应用

  WB: 1:5,000
  IP: 1:10-1:100

• 

  Fig1: Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

  

  Fig2: Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  Lane 1: N-terminal FLAG-tagged recombinant protein
  Lane 2: C-terminal FLAG-tagged recombinant protein

• 背景文献

• 暂无

• Publishing research using Anti-DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

• PMID:
  36597142 (Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota Author: Wang, J., Ding, Y., Li, D., Zhu, N., Nishiyama, A., & Yuan, Y. Published: 2023 Application: WB Species: Human Journal: Cell Commun Signal. IF: 7.525
• PMID:
  37220310 Discovery of a Selective and Orally Bioavailable FGFR2 Degrader for Treating Gastric Cancer Author: Ma, L., Li, Y., Luo, R., Wang, Y., Cao, J., Fu, W., Qian, B., Zheng, L., Tang, L., Lv, X., Zheng, L., Liang, G., & Chen, L. Published: 2023 Application: WB Species: Mouse,Human Journal: J Med Chem IF: 8.039
• PMID:
  36298827 The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins Author: Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Journal: Viruses. IF: 5.818
• PMID:
  35266845 TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation Author: Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J. Published: 2022 Application: WB Species: Chicken Journal: Autophagy IF: 16.016
• PMID:
  35632681 Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins Author: Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Species: Human Journal: Viruses IF: 5.048
• PMID:
  35283818 Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein Author: Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Species: Human Journal: Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein IF: 5.640
• PMID:
  36084138 TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus Author: Zhao, L., Liu, Q., Huang, J., Lu, Y., Zhao, Y., & Ping, J. Published: 2022 Application: WB Species: Human Journal: PLoS Pathog. IF: 7.464
• PMID:
  35251046 BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection Author: Zhao, S., Chen, G., Kong, X., Chen, N., & Wu, X. Published: 2022 Journal: Front Immunol IF: 7.561
• PMID:
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• PMID:
  33975961 Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21. Author: Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J. Published: 2021 Application: WB Journal: mSystems IF: 6.469
• PMID:
  34113359 Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315. Author: Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H. Published: 2021 Application: WB Species: Nicotiana tabacum Journal: Front Plant Sci IF: 5.753
• PMID:
  34745055 The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1 Author: Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J. Published: 2021 Application: IP Journal: Front Microbiol IF: 5.81
• PMID:
  33413620 The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4. Author: Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J. Published: 2021 Application: WB Journal: Veterinary Research IF: 1.792
• PMID:
  33879767 ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346. Author: Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H. Published: 2021 Application: WB Species: Human Journal: Nat Commun IF: 12.12
• PMID:
  34372697 DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication Author: Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J. Published: 2021 Application: WB Species: Human Journal: mBio IF: 7.861
• PMID:
  32467610 The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs Author: Jian Chen;Fengdong Zhao Published: 2020 Application: WB,IP Species: human Journal: Experimental & Molecular Medicine IF: 4.743
• PMID:
  33392195 Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function Author: Guoqiang Xu;Xiangao Huang Published: 2020 Application: IP Species: human Journal: Front Cell Dev Biol. IF: 5.201
• PMID:
  33003329 Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus Author: Jihui Ping Published: 2020 Application: COIP Species: human Journal: Viruses IF: 3.816
• PMID:
  33073687 Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid Author: Jinyan Gu; Jiyong Zhou Published: 2020 Application: WB,CoIP Species: human Journal: VIRULENCE IF: 5.542
• PMID:
  32937100 Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation Author: Di Wang Published: 2020 Application: WB,IP Species: mice Journal: Molecular Cell IF: 15.584
• PMID:
  32582087 Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins Author: Jiyong Zhou Published: 2020 Application: WB Species: pig Journal: Front Microbiol. IF: 4.235
• PMID:
  30767704 TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction Author: Wei Liu Published: 2019 Application: WB Species: HEK293 cells Journal: Autophagy. IF: 11.059
• PMID:
  30396897 Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and StressActivated Protein Kinase 1-Dependent Manner Author: Qin Liu Published: 2019 Application: WB Species: Escherichia coli Journal: Infection and Immunity IF: 3.256
• PMID:
  31857589 Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress Author: Wei Liu;Chun Tang Published: 2019 Application: IP,CO-IP Species: human Journal: NATURE COMMUNICATIONS IF: 11.878
• PMID:
  30366764 Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages Author: 王迪 Published: 2018 Application: WB Species: None Journal: Immunity. IF: 19.734
• PMID:
  30018336 Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop Author: Jihong Sun6 & Xiaojian Wang1 Published: 2018 Application: WB Species: mouse Journal: Nat Commun IF: 12.353
• PMID:
  29653076 E4bp4 Regulates Carboxylesterase 2 Enzymes through Repression of the Nuclear Receptor Rev-erbα in Mice Author: Baojian Wu Published: 2018 Application: WB Species: mice Journal: Biochemical Pharmacology IF: 4.581
• PMID:
  26704979 Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents Author: Kaifu T Published: 2016 Application: WB Species: human Journal: Nucleic Acids Research IF: 9.2

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.