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概述
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产品描述
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
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产品名称
Anti-DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody
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验证应用
WB,IP
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抗体类型
兔多抗
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免疫原
Synthetic peptide immune sequence is CDYKDDDDK.
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偶联
Non-conjugated
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RRID
AB_3068715
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性能
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形态
Liquid
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浓度
1 mg/mL.
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存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
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亚型
IgG
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纯化方式
Immunogen affinity purified.
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其它名称
- DDDDK epitope tag antibody
- DDDK antibody
- ddk antibody
- DYKDDDDK antibody
- DYKDDDDK epitope tag antibody
- DYKDDDDK tag antibody
- ECS epitope tag antibody
- ECS tag antibody
- Enterokinase Cleavage Site epitope tag antibody
- Enterokinase Cleavage Site tag antibody
- FLAG antibody
- FLAG tag antibody
- DYKDDDDKTag(FLAG)
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应用
WB: 1:5,000
IP: 1:10-1:100
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Fig1: Western blot analysis of DYKDDDDK Tag (FLAG) on DYKDDDDK Tag recombinant protein (80kDa). Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of DYKDDDDK Tag (FLAG) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0912-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: N-terminal FLAG-tagged recombinant protein
Lane 2: C-terminal FLAG-tagged recombinant protein
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Publishing research
using Anti-DYKDDDDK Tag (FLAG) Rabbit Polyclonal Antibody? Please let us know so
that we can cite the reference in this datasheet.
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PMID:
36597142
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
Author: Wang, J., Ding, Y., Li, D., Zhu, N., Nishiyama, A., & Yuan, Y. Published: 2023 Application: WB Species: Human Journal: Cell Commun Signal. IF: 7.525
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PMID:
37220310
Discovery of a Selective and Orally Bioavailable FGFR2 Degrader for Treating Gastric Cancer
Author: Ma, L., Li, Y., Luo, R., Wang, Y., Cao, J., Fu, W., Qian, B., Zheng, L., Tang, L., Lv, X., Zheng, L., Liang, G., & Chen, L. Published: 2023 Application: WB Species: Mouse,Human Journal: J Med Chem IF: 8.039
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PMID:
36298827
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
Author: Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Journal: Viruses. IF: 5.818
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PMID:
35266845
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
Author: Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J. Published: 2022 Application: WB Species: Chicken Journal: Autophagy IF: 16.016
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PMID:
35632681
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
Author: Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Species: Human Journal: Viruses IF: 5.048
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PMID:
35283818
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
Author: Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J. Published: 2022 Application: WB Species: Human Journal: Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein IF: 5.640
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PMID:
36084138
TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
Author: Zhao, L., Liu, Q., Huang, J., Lu, Y., Zhao, Y., & Ping, J. Published: 2022 Application: WB Species: Human Journal: PLoS Pathog. IF: 7.464
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PMID:
35251046
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
Author: Zhao, S., Chen, G., Kong, X., Chen, N., & Wu, X. Published: 2022 Journal: Front Immunol IF: 7.561
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PMID:
35806389
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
Author: Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C. Published: 2022 Application: WB Journal: Int J Mol Sci. IF: 5.924
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PMID:
33975961
Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.
Author: Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J. Published: 2021 Application: WB Journal: mSystems IF: 6.469
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PMID:
34113359
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
Author: Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H. Published: 2021 Application: WB Species: Nicotiana tabacum Journal: Front Plant Sci IF: 5.753
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PMID:
34745055
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
Author: Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J. Published: 2021 Application: IP Journal: Front Microbiol IF: 5.81
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PMID:
33413620
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
Author: Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J. Published: 2021 Application: WB Journal: Veterinary Research IF: 1.792
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PMID:
33879767
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
Author: Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H. Published: 2021 Application: WB Species: Human Journal: Nat Commun IF: 12.12
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PMID:
34372697
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
Author: Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J. Published: 2021 Application: WB Species: Human Journal: mBio IF: 7.861
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PMID:
32467610
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs
Author: Jian Chen;Fengdong Zhao Published: 2020 Application: WB,IP Species: human Journal: Experimental & Molecular Medicine IF: 4.743
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PMID:
33392195
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
Author: Guoqiang Xu;Xiangao Huang Published: 2020 Application: IP Species: human Journal: Front Cell Dev Biol. IF: 5.201
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PMID:
33003329
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
Author: Jihui Ping Published: 2020 Application: COIP Species: human Journal: Viruses IF: 3.816
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PMID:
33073687
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
Author: Jinyan Gu; Jiyong Zhou Published: 2020 Application: WB,CoIP Species: human Journal: VIRULENCE IF: 5.542
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PMID:
32937100
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
Author: Di Wang Published: 2020 Application: WB,IP Species: mice Journal: Molecular Cell IF: 15.584
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PMID:
32582087
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins
Author: Jiyong Zhou Published: 2020 Application: WB Species: pig Journal: Front Microbiol. IF: 4.235
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PMID:
30767704
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
Author: Wei Liu Published: 2019 Application: WB Species: HEK293 cells Journal: Autophagy. IF: 11.059
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PMID:
30396897
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and StressActivated Protein Kinase 1-Dependent Manner
Author: Qin Liu Published: 2019 Application: WB Species: Escherichia coli Journal: Infection and Immunity IF: 3.256
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PMID:
31857589
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress
Author: Wei Liu;Chun Tang Published: 2019 Application: IP,CO-IP Species: human Journal: NATURE COMMUNICATIONS IF: 11.878
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PMID:
30366764
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages
Author: 王迪 Published: 2018 Application: WB Species: None Journal: Immunity. IF: 19.734
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PMID:
30018336
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop
Author: Jihong Sun6 & Xiaojian Wang1 Published: 2018 Application: WB Species: mouse Journal: Nat Commun IF: 12.353
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PMID:
29653076
E4bp4 Regulates Carboxylesterase 2 Enzymes through Repression of the Nuclear Receptor Rev-erbα in Mice
Author: Baojian Wu Published: 2018 Application: WB Species: mice Journal: Biochemical Pharmacology IF: 4.581
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PMID:
26704979
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents
Author: Kaifu T Published: 2016 Application: WB Species: human Journal: Nucleic Acids Research IF: 9.2
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Specific Protocols
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you
get on.
Click here to view the
general protocols