• 概述

• 产品描述 Cytokeratin 5 is commonly found in the cells on the outermost layer of skin in humans and animals. It is encoded by the KRT5 gene and pairs with the type I keratin K14. Cytokeratin 5 has become an important biomarker for different types of cancer, including mesothelioma, breast cancer and lung cancer. It helps differentiate squamous carcinomas from adenocarcinomas. Pathologists use cytokeratin 5 to distinguish mesothelioma from adenocarcinoma, the most common type of lung cancer. Cytokeratin 5/6 cannot identify mesothelioma on its own. Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers. Pathologists use cytokeratin 5/6 to stain cancer tissue samples. Cytokeratin 5/6 immunoreactivity is rarely seen in adenocarcinomas of the lung. If a tumor sample shows strong expression of cytokeratin 5/6, it gives pathologists a hint the tumor is malignant mesothelioma rather than a metastatic adenocarcinoma. However, this marker is not effective for all cell types of mesothelioma. Cytokeratin 5/6 staining is usually weak or negative for sarcomatoid mesothelioma, the least common and hardest-to-treat cell type of the asbestos-related cancer. The marker is also not effective in telling the difference between pleural mesothelioma and squamous cell carcinomas. About 25 to 30 percent of all lung cancers are squamous cell carcinomas.
• 产品名称 iFluor™ 647 Conjugated Anti-Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]
• 分子量 Predicted band size: 62 kDa
• 种属反应性 Human
• 验证应用 IF-Cell,IF-Tissue
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within human Cytokeratin 5 aa 70-110.
• 偶联 iFluor™ 647

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm.
• 数据链接 SwissProt: P13647 Human
  
• 其它名称
  • 58 kDa cytokeratin antibody
  • CK-5 antibody
  • CK5 antibody
  • Cytokeratin-5 antibody
  • Cytokeratin5 antibody
  • DDD antibody
  • DDD1 antibody
  • EBS2 antibody
  • epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
  • K2C5_HUMAN antibody
  • K5 antibody
  • keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) antibody
  • Keratin 5 antibody
  • Keratin antibody
  • keratin complex 2, basic, gene 5 antibody
  • keratin, type II cytoskeletal 5 antibody
  • Keratin-5 antibody
  • Keratin5 antibody
  • KRT 5 antibody
  • Krt5 antibody
  • KRT5A antibody
  • type II cytoskeletal 5 antibody
  • Type-II keratin Kb5 antibody
  • iFluor™647ConjugatedAnti-Cytokeratin5
  more

• 应用

  IF-Cell: 1:100
  IF-Tissue: 1:50

• 

  Fig1: Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720126F) and Vimentin (EM0401).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720126F, red) at 1/50 dilution and Vimentin (EM0401, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
  
  iFluor™ 488 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

  

  Fig2: Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (HA720126F) at 1/100 dilution.
  
  Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (HA720126F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) were used as the secondary antibody at 1/800 dilution.

• 背景文献

• 1. Colopy SA. et al. A population of progenitor cells in the basal and intermediate layers of the murine bladder urothelium contributes to urothelial development and regeneration. Dev Dyn 243:988-98 (2014).
  
  2. Wang J. et al. Symmetrical and asymmetrical division analysis provides evidence for a hierarchy of prostate epithelial cell lineages. Nat Commun 5:4758 (2014).

• Publishing research using iFluor™ 647 Conjugated Anti-Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.