• 概述

• 产品描述 Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells and have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. For example, many types of cancer cells express Cytokeratin 19 (CK19), an epithelial cytoskeletal protein within the suprabasal squamous epithelium. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma. Cytokeratin 19 fragment levels in serum have been documented as a marker for lung cancer. Clinical investigations have suggested that serum CYFRA 21-1, a fragment of Cytokeratin 19, may be among the most useful tumor markers.
• 产品名称 iFluor™ 594 Conjugated Anti-Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody [SA30-06]
• 分子量 Predicted band size: 44 kDa
• 种属反应性 Human,Mouse
• 验证应用 IF-Cell,IF-Tissue,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within Human Cytokeratin 19 aa 348-400.
• 偶联 iFluor™ 594

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoskeleton.
• 数据链接 SwissProt: P08727 Human
  
• 其它名称
  • 40 kDa keratin intermediate filament antibody
  • CK 19 antibody
  • CK-19 antibody
  • CK19 antibody
  • Cytokeratin 19 antibody
  • Cytokeratin-19 antibody
  • K19 antibody
  • K1C19_HUMAN antibody
  • K1CS antibody
  • Keratin 19 antibody
  • Keratin type I 40 kD antibody
  • Keratin type I 40kD antibody
  • Keratin type I cytoskeletal 19 antibody
  • Keratin, type I cytoskeletal 19 antibody
  • Keratin, type I, 40 kd antibody
  • Keratin-19 antibody
  • KRT19 antibody
  • MGC15366 antibody
  • iFluor™594ConjugatedAnti-Cytokeratin19
  more

• 应用

  IF-Cell: 1:50
  IF-Tissue: 1:100
  FC: 1ug/mL

• 

  Fig1: Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19.
  Cells were fixed in 4% paraformaldehyde and permeabilized with 0.05% Triton X-100 in PBS for 10 minutes, and then blocked with 2% negative goat serum for 15 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720115F at 1/50 dilution Rabbit monoclonal to Cytokeratin 19 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

  

  Fig2: Immunocytochemistry analysis of SK-Br-3 cells labeling Cytokeratin 19.
  Cells were fixed in 100% methanol and permeabilized with 0.1% Triton X-100 in PBS for 10 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. The cells were then incubated overnight at +4℃ with HA720115F at 1/50 dilution Rabbit monoclonal to Cytokeratin 19 (iFluor™ 594)(shown in red). DAPI was used as nuclear counterstain.

  

  Fig3: Immunofluorescence analysis of paraffin-embedded human breast tissue labeling Cytokeratin 19 (HA720115F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720115F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  

  Fig4: Immunofluorescence analysis of paraffin-embedded human kidney tissue labeling Cytokeratin 19 (HA720115F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 19 (HA720115F, iFluor™ 594) at 1/100 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

  

  Fig5: Flow cytometric analysis of MCF-7 cells labeling Cytokeratin 19.
  
  Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720115F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  

  Fig6: Flow cytometric analysis of SK-Br-3 cells labeling Cytokeratin 19.
  
  Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with Cytokeratin 19 (HA720115F, red, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. Guye P. et. al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016).
  
  2. Cui M. et. al. PTEN is a potent suppressor of small cell lung cancer. Mol Cancer Res 12:654-9 (2014).

• Publishing research using iFluor™ 594 Conjugated Anti-Cytokeratin 19 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.