• 概述

• 产品描述 Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of Cytokeratin 14. The gene encoding human Cytokeratin 14 maps to chromosome 17q12-21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.
• 产品名称 iFluor™ 647 Conjugated Anti-Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody [SC65-06]
• 分子量 Predicted band size: 52 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 IF-Cell,IF-Tissue
• 抗体类型 重组兔单抗
• 免疫原 Recombinant protein within Human Cytokeratin 14 aa 250-484.
• 偶联 iFluor™ 647

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cytoplasm, Nucleus.
• 数据链接 SwissProt: P02533 Human
  SwissProt: Q6IFV1 Rat
  
• 其它名称
  • CK 14 antibody
  • CK-14 antibody
  • ck14 antibody
  • Cytokeratin 14 antibody
  • Cytokeratin-14 antibody
  • Cytokeratin14 antibody
  • Dowling Meara antibody
  • EBS3 antibody
  • EBS4 antibody
  • Epidermolysis bullosa simplex antibody
  • K14 antibody
  • K1C14_HUMAN antibody
  • Keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner) antibody
  • Keratin 14 antibody
  • Keratin antibody
  • Keratin type I cytoskeletal 14 antibody
  • Keratin, type I cytoskeletal 14 antibody
  • Keratin-14 antibody
  • Keratin14 antibody
  • Koebner antibody
  • Krt 14 antibody
  • Krt14 antibody
  • NFJ antibody
  • OTTHUMP00000164624 antibody
  • type I cytoskeletal 14 antibody
  • iFluor™647ConjugatedAnti-Cytokeratin14
  more

• 应用

  IF-Cell: 1:100
  IF-Tissue: 1:400

• 

  Fig1: Immunocytochemistry analysis of A431 cells labeling Cytokeratin 14 with Rabbit anti-Cytokeratin 14 antibody (HA720147F) at 1/100 dilution.
  
  Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37℃. Cells were then incubated with Rabbit anti-Cytokeratin 14 antibody (HA720147F, red) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, green) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/800 dilution.

  

  Fig2: Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 (HA720147F), Cytokeratin 10 (HA720134F) and Vimentin (EM0401).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 14 (HA720147F, red) at 1/400 dilution, Cytokeratin 10 (HA720134F, green) at 1/400 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
  
  Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG (HA1125) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

• 背景文献

• 1. Pastar I. et al. Interactions of methicillin resistant Staphylococcus aureus USA300 and Pseudomonas aeruginosa in polymicrobial wound infection. PLoS One 8:e56846 (2013).
  
  2. DeWard AD. et al. Cellular heterogeneity in the mouse esophagus implicates the presence of a nonquiescent epithelial stem cell population. Cell Rep 9:701-11 (2014).

• Publishing research using iFluor™ 647 Conjugated Anti-Cytokeratin 14 Recombinant Rabbit Monoclonal Antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.