• 概述

• 产品描述 This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
• 产品名称 iFluor™ 488 Conjugated Anti-Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
• 分子量 Predicted band size: 109 kDa
• 种属反应性 Human,Mouse,Rat
• 验证应用 IF-Cell,IF-Tissue
• 抗体类型 重组兔单抗
• 免疫原 Recombinant protein within Human Collagen VI aa 17-255 / 1,028.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Extracellular matrix.
• 数据链接 SwissProt: P12109 Human
  SwissProt: P12110 Human
  SwissProt: P12111 Human
  SwissProt: Q02788 Mouse
  SwissProt: Q04857 Mouse
  
• 其它名称
  • Alpha 1 (VI) chain (61 AA) antibody
  • CO6A1_HUMAN antibody
  • COL6A1 antibody
  • COL6A2 antibody
  • COL6A3 antibody
  • Collagen alpha 2(VI) chain antibody
  • Collagen alpha 3(VI) chain antibody
  • Collagen alpha-1(VI) chain antibody
  • Collagen type VI alpha 1 antibody
  • Collagen type VI alpha 2 antibody
  • Collagen type VI alpha 3 antibody
  • Collagen VI alpha 1 polypeptide antibody
  • Collagen VI alpha 2 polypeptide antibody
  • Collagen VI alpha 3 polypeptide antibody
  • CollagenVI antibody
  • Human mRNA for collagen VI alpha 2 C terminal globular domain antibody
  • OPLL antibody
  • PP3610 antibody
  • iFluor™488ConjugatedAnti-CollagenVI
  more

• 应用

  IF-Cell: 1:100
  IF-Tissue: 1:50

• 

  Fig1: Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  

  Fig2: Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F).
  
  The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.

• 背景文献

• 1. Kristofik N et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016).
  
  2. Okawa S et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol 28:37-41 (2015).

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.