• 概述

• 产品描述 Calbindin D28K and Calretinin (also designated CR or 29 kDa Calbindin) are two closely related intracellular calcium-binding proteins belonging to the Troponin-C superfamily. Initially isolated from chick retina, Calretinin shares 58% identical residues with human Calbindin D28K. Calretinin is expressed in the brain and is particularly abundant in auditory neurons with precisely timed discharges. Neurons in the nucleus accumbens containing Calretinin all possess nuclear indentations. Calretinin-immunoreactive boutons form asymmetrical and symmetrical synaptic specializations on spines, dendrites and somata. The symmetrical synaptic specializations have medium-sized spiny neurons and contact other Calretinin-immunoreactive somata. Calretinin is widely used as a immunocytochemical marker for mesothelioma.
• 产品名称 iFluor™ 488 Conjugated Anti-Calretinin Recombinant Rabbit Monoclonal Antibody [JM12-93]
• 分子量 Predicted band size: 32 kDa
• 种属反应性 Human
• 验证应用 IF-Cell,FC
• 抗体类型 重组兔单抗
• 免疫原 Synthetic peptide within human Calretinin aa 60-100.
• 偶联 iFluor™ 488

• 性能

• 形态 Liquid
• 浓度 1 mg/mL.
• 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液 Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
• 亚型 IgG
• 纯化方式 Protein A affinity purified.
• 亚细胞定位 Cuticular plate, cytosol, nucleus, synaptic membrane, dendrite, gap junction, neuron projection, parallel fiber to Purkinje cell synapse, stereocilium, synapse, terminal bouton.
• 数据链接 SwissProt: P22676 Human
  
• 其它名称
  • 29 kDa calbindin antibody
  • CAB 29 antibody
  • CAB29 antibody
  • CAL 2 antibody
  • CAL2 antibody
  • CALB 2 antibody
  • CALB2 antibody
  • CALB2_HUMAN antibody
  • Calbindin 2 29kDa antibody
  • Calbindin 2 antibody
  • Calbindin D29K antibody
  • Calbindin2 antibody
  • Calretinin antibody
  • CR antibody
  • iFluor™488ConjugatedAnti-Calretinin
  more

• 应用

  IF-Cell: 1:100
  FC: 1:500-1:1,000

• 

  Fig1: Immunocytochemistry analysis of SH-SY5Y cells labeling Calretinin with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution.
  
  Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Calretinin antibody (HA720153F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
  
  Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.

  

  Fig2: Flow cytometric analysis of SH-SY5Y cells labeling Calretinin.
  
  Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with Calretinin (HA720153F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• 背景文献

• 1. Francavilla C et al. Phosphoproteomics of Primary Cells Reveals Druggable Kinase Signatures in Ovarian Cancer. Cell Rep 18:3242-3256 (2017).
  
  2. McMahon SM et al. Multiple cytosolic calcium buffers in posterior pituitary nerve terminals. J Gen Physiol 147:243-54 (2016).

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.