HA-tag is derived from the human influenza hemagglutinin (HA) molecule corresponding to amino acids 98-106. and is commonly expressed as a tag in conjunction with proteins to aid in identification and purification of the tagged protein for functional analysis. This antibody recognizes the HA-tag sequence YPYDVPDYA at the N-terminus, C-terminus or internal site of fusion protein.
N-terminus, C-terminus or internal site of HA fusion protein
PBS PH=7.5, 10mg/ml BSA, 100mM trehalose, 50% glycerol
1mg/ml VHH-HRP conjugate
store at –20 °C, Protect from light
WB 1 : 2,000-1 : 10,000
ELISA 1 : 5,000-1 : 20,000
Western blot analysis of Anti-HA-HRP with HA tag-tagged Recombinant protein at 20ng per lane.
Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (NB003H) was used in 5% BSA at room temperature for 2 hours at 1 in 2,000 dilution (Lane 1) and 1 in 10,000 dilution (Lane 2) respectively.
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