NUP43 Rabbit Polyclonal Antibody
Catalog# ER1914-25
NUP43 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
概述
产品名称
NUP43 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human NUP43 1-100/380
种属反应性
Human, Mouse
验证应用
WB, IHC-P, IF-Tissue
分子量
42 KDa
偶联
unconjugated
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
靶点
功能
NUP43 probably mediates the assembly of subdomains of the nuclear pore complex (NPC) or facilitates the interaction of transport complexes with the NPC.
背景文献
暂无
亚细胞定位
Nucleus; nuclear pore complex.
UNIPROT
别名
bA350J20.1 antibody
FLJ13287 antibody
Nucleoporin 43kDa antibody
Nucleoporin Nup43 antibody
Nup107-160 subcomplex subunit Nup43 antibody
p42 antibody
图片
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Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti-NUP43 (ER1914-25) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 30 kD -
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NUP43) Polyclonal Antibody, Unconjugated (ER1914-25) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"