SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
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Catalog# HA721316
SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
概述
产品名称
SIRP alpha Recombinant Rabbit Monoclonal Antibody [JE36-20]
抗体类型
Recombinant Rabbit monoclonal Antibody
免疫原
Recombinant protein within Human SIRP alpha aa 395-504 (Cytoplasmic).
种属反应性
Human, Mouse, Rat
验证应用
WB, IHC-P, FC
分子量
Predicted band size: 55 kDa
阳性对照
THP-1 cell lysate, HL-60 cell lysate, mouse brain tissue lysate, human lymph node tissue, human spleen tissue.
偶联
unconjugated
克隆号
JE36-20
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:1,000
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IHC-P
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1:1,000
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FC
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1:1,000
靶点
功能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. Binds PTPN11 when tyrosine-phosphorylated, except in macrophages, where it primarily binds PTPN6. Binds GRB2 in vitro. Binds FGR. Binds JAK2 irrespective of its phosphorylation status and forms a stable complex. Binds SCAP1 and/or SCAP2. The resulting complex recruits FYB1. Binds PTK2B.
背景文献
1. Timms J.F., Swanson K.D., Marie-Cardine A., Raab M., Rudd C.E., Schraven B., Neel B.G. SHPS-1 is a scaffold for assembling distinct adhesion-regulated multi- protein complexes in macrophages. Curr. Biol. 9:927-930 (1999).
2. Latour S., Tanaka H., Demeure C., Mateo V., Rubio M., Brown E.J., Maliszewski C., Lindberg F.P., Oldenborg A., Sarfati M. Bidirectional negative regulation of human T and dendritic cells by CD47 and its cognate receptor signal-regulator protein-alpha: down-regulation of IL-12 responsiveness and inhibition of dendritic cell activation. J. Immunol. 167:2547-2554 (2001)
亚细胞定位
Membrane
别名
Signal regulatory protein alpha type 1 antibody
Bit antibody
Brain Ig like molecule with tyrosine based activation motifs antibody
Brain Ig-like molecule with tyrosine-based activation motifs antibody
Brain immunoglobulin like molecule with tyrosine based activation motifs antibody
CD172 antigen like family member A antibody
CD172 antigen-like family member A antibody
CD172a antibody
CD172a antigen antibody
Inhibitory receptor SHPS-1 antibody
展开图片
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☑ Knockdown (KD)
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-SIRP alpha KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 75-100 kDa
Exposure time: 9 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721316) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of SIRP alpha on different lysates with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate (10 µg/Lane)
Lane 2: HL-60 cell lysate (10 µg/Lane)
Lane 3: Mouse brain tissue lysate (20 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 75-100 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721316) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-SIRP alpha antibody (HA721316) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721316) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of THP-1 cells labeling SIRP alpha.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721316, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human
Conjugate: unconjugated
