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Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

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Catalog# HA210126

Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

This product has been cited in peer reviewed publications, see list HERE

Product Information

Product name

Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein

Catalog No.

HA210126

Predicted molecular mass

The protein has a predicted MW of 114 kDa (ITGAV)&81.3 kDa (ITGB3). Due to glycosylation,the protein migrates to 85-140 kDa based on Tris-Bis PAGE result.

Protein construction description

Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Phe31-Val992(ITGAV) acidic tail&GLy27-Asp718(ITGB3) basic tail.

Accession

P05106-1(ITGB3)

Source

HEK293

Purity

> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC

Endotoxin

Less than 1EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Species

Human

Background

ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling.

Usage guide

Storage

-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

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