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Human IL-1R3/IL-1 RAcP Protein

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Catalog# HA210101

Human IL-1R3/IL-1 RAcP Protein

Product Information

Product name

Human IL-1R3/IL-1 RAcP Protein

Catalog No.

HA210101

Predicted molecular mass

The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result.

Protein construction description

Recombinant Human IL-1R3/IL-1 RAcP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ser21-Glu359.

Accession

Q9NPH3-1

Source

HEK293

Purity

> 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC

Endotoxin

Less than 1EU per μg by the LAL method.

Formulation

Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Species

Human

Background

IL-1 Receptor Accessory Protein (IL-1 RAcP), also known as IL-1 R3, is a ubiquitously expressed 70-90 kDa member of the Interleukin-1 receptor family of proteins. It serves as a non-ligand-binding component of the receptors for IL-1 alpha, IL-1 beta, IL-33, and IL-36. IL-1 R3 is a coreceptor for IL1RL2 in the IL-36 signaling system. IL-1 R3 is a coreceptor with IL1R1 in the IL-1 signaling system. Associates with IL1R1 bound to IL1B to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B and other pathways.

Usage guide

Storage

-20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3-6 months after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Reconstitution

Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

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