Hepsin/TMPRSS1 Rabbit Polyclonal Antibody
Catalog# ER1910-49
Hepsin/TMPRSS1 Rabbit Polyclonal Antibody
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WB
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IHC-P
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IF-Tissue
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Human
概述
产品名称
Hepsin/TMPRSS1 Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
KLH conjugated synthetic peptide derived from human Hepsin 311-417/417
种属反应性
Human
验证应用
WB, IHC-P, IF-Tissue
分子量
28 KDa
阳性对照
Present in most tissues, with the highest level in liver.
偶联
unconjugated
产品特性
形态
Liquid
存放说明
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
存储缓冲液
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
亚型
IgG
纯化方式
Protein A affinity purified.
应用稀释度
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WB
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1:500-2000
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IHC-P
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1:100-500
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IF-tissue
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1:100-500
靶点
功能
Plays an essential role in cell growth and maintenance of cell morphology. May mediate the activating cleavage of HGF and MST1/HGFL.
背景文献
暂无
亚细胞定位
Membrane; Single-pass type II membrane protein.
UNIPROT
别名
EC 3.4.21. antibody
HEPS antibody
HEPS_HUMAN antibody
Hepsin antibody
Hpn antibody
Serine protease hepsin antibody
Serine protease hepsin catalytic chain antibody
Serine protease hepsin non catalytic chain antibody
TMPRSS 1 antibody
TMPRSS1 antibody
展开图片
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Sample: HepG2 (human)Cell Lysate at 40 ug
Primary: Anti- Hepsin/TMPRSS1 (ER1910-49)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28kD
Observed band size: 28 kD -
Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Hepsin Polyclonal Antibody, Unconjugated(ER1910-49) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
请注意: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"