GFP Rabbit Polyclonal Antibody
Catalog# R1312-2
GFP Rabbit Polyclonal Antibody
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WB
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ELISA
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IP
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Species independent
概述
产品名称
GFP Rabbit Polyclonal Antibody
抗体类型
Rabbit Polyclonal Antibody
免疫原
Recombinant full length protein of Aequorea victoria GFP.
种属反应性
Species independent
验证应用
WB, ELISA, IP
分子量
Predicted band size: 26 kDa
阳性对照
GFP protein
偶联
unconjugated
RRID
产品特性
形态
Liquid
浓度
1ug/ul
存放说明
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型
IgG
纯化方式
Immunogen affinity purified.
应用稀释度
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WB
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1:10,000
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ELISA
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1:10,000
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IP
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2-5 µg/ml.
靶点
功能
Green fluorescence protein (GFP) is derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. It has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without chemical staining.
背景文献
1. "Primary structure of the Aequorea victoria green-fluorescent protein."Prasher D.C., Eckenrode V.K., Ward W.W., Prendergast F.G., Cormier M.J.Gene 111:229-233(1992)
2. "A molecular thermometer based on fluorescent protein blinking."Wong F.H., Banks D.S., Abu-Arish A., Fradin C.J. Am. Chem. Soc. 129:10302-10303(2007)
序列相似性
Belongs to the GFP family.
组织特异性
Photocytes.
翻译后修饰
Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
UNIPROT #
别名
GFP antibody
Green fluorescent protein antibody
yfp antibody
图片
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Western blot analysis on GFP protein using rabbit polyclonal antibody.
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GFP tag was immunoprecipitated in 5µg GFP Tag fusion protein lysate with R1312-2 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1004-8 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.
Lane 1: GFP Tag fusion protein lysate (input).
Lane 2: Rabbit IgG instead of R1312-2 in GFP Tag fusion protein lysate.
Lane 3: ET1602-7 IP in GFP Tag fusion protein lysate.
Lane 4: ET1604-25 IP in GFP Tag fusion protein lysate.
Lane 5: ET1604-26 IP in GFP Tag fusion protein lysate.
Lane 6: ET1607-31 IP in GFP Tag fusion protein lysate.
Lane 7: R1312-2 IP in GFP Tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"