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Anti-Dnmt1 antibody [JF09-89]

重组兔单抗 | 货号: ET1702-77


规格:
价格:RMB   2500
  • 概述

  • 产品描述 Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
  • 产品名称 Anti-Dnmt1 antibody [JF09-89]
  • 分子量 183 kDa
  • 种属反应性 Human,Mouse,Rat
  • 验证应用 WB,ICC/IF,IHC
  • 抗体类型 重组兔单抗
  • 免疫原 recombinant protein
  • 偶联 Non-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 1 mg/mL.
  • 存放说明 Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • 存储缓冲液 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • 亚型 IgG
  • 纯化方式 Protein A purified.
  • 亚细胞定位 Nucleus.
  • 数据链接 P26358 Human
    P13864 Mouse
    Q9Z330 Rat
  • 其它名称 ADCADN antibody
    AIM antibody
    CXXC finger protein 9 antibody
    CXXC-type zinc finger protein 9 antibody
    CXXC9 antibody
    DNA (cytosine 5 ) methyltransferase 1 antibody
    DNA (cytosine-5)-methyltransferase 1 antibody
    DNA methyltransferase 1 antibody
    DNA methyltransferase HsaI antibody
    DNA methyltransferase M.HsaI. antibody
    DNA MTase antibody
    DNA MTase HsaI antibody
    DNMT 1 antibody
    DNMT antibody
    Dnmt1 antibody
    DNMT1_HUMAN antibody
    Dnmt1o antibody
    FLJ16293 antibody
    HSN1E antibody
    M.HsaI antibody
    MCMT antibody
    Met1 antibody
    MGC104992 antibody
    mMmul antibody
    MommeD2 antibody
  • 应用

    WB: 1:1,000-1:2,000
    ICC: 1:50-1:200
    IHC: 1:50-1:200

  • 背景文献

  • 1. Liu R et al. Dnmt1 regulates the myogenic lineage specification of muscle stem cells. Sci Rep 6:35355 (2016).
    2. Chalertpet K et al. Human papillomavirus type 16 E7 oncoprotein mediates CCNA1 promoter methylation. Cancer Sci 106:1333-40 (2015).
  • Publishing research using Anti-Dnmt1 antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols