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Anti-PUF60 antibody

兔多抗 | 货号: ER1915-62


规格:
价格:RMB   2500
  • 概述

  • 产品描述 DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Does not repress TFIIH-mediated transcription in xeroderma pigmentosum complementation group B (XPB) cells. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Isoform 6 failed to repress MYC transcription and inhibited FIR-induced apoptosis in colorectal cancer. Isoform 6 may contribute to tumor progression by enabling increased MYC expression and greater resistance to apoptosis in tumors than in normal cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA; binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with TROVE2. Binds to poly(U) RNA.
  • 产品名称 Anti-PUF60 antibody
  • 分子量 60 KDa
  • 种属反应性 Human,Mouse,Rat
  • 验证应用 WB,IHC-P
  • 抗体类型 兔多抗
  • 免疫原 KLH conjugated synthetic peptide derived from human PUF60 1-100/559
  • 偶联 Non-conjugated
  • 性能

  • 形态 Liquid
  • 浓度 1 mg/mL.
  • 存放说明 Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • 存储缓冲液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • 亚型 IgG
  • 纯化方式 affinity purified by Protein A
  • 亚细胞定位 Nucleus. Colocalizes partially with TROVE2.
  • 数据链接 SwissProt: Q9UHX1 Human
  • 其它名称 60 kDa poly(U)-binding-splicing factor
    FBP-interacting repressor
    FIR
    FLJ31379
    FUSE-binding protein-interacting repressor
    Poly U binding splicing factor 60KDa
    Poly(U)-binding-splicing factor PUF60
    poly-U binding splicing factor PUF60
    PUF60
    PUF60_HUMAN
    pyrimidine tract binding splicing factor
    Ro ribonucleoprotein-binding protein 1
    Ro-binding protein 1
    RoBP1
    RoBPI
    siah binding protein 1
    Siah-binding protein 1
    Siah-BP1
    SIAHBP1.
  • 应用

    WB:1:500-2000
    IHC-P:1:400-800

  • 背景文献

  • 暂无
  • Publishing research using Anti-PUF60 antibody? Please let us know so that we can cite the reference in this datasheet.

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Specific Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols