The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120.
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 25%Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified
CD 4 antibody
CD4 (L3T4) antibody
CD4 antigen (p55) antibody
CD4 antigen antibody
CD4 molecule antibody
CD4 receptor antibody
CD4+ Lymphocyte deficiency, included antibody
Lymphocyte antigen CD4 antibody
T cell antigen T4 antibody
T cell antigen T4/LEU3 antibody
T cell differentiation antigen L3T4 antibody
T cell OKT4 deficiency, included antibody
T cell surface antigen T4/Leu 3 antibody
T cell surface antigen T4/Leu3 antibody
T cell surface glycoprotein CD4 antibody
T-cell surface antigen T4/Leu-3 antibody
T-cell surface glycoprotein CD4 antibody
W3/25 antigen antibody
Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.
Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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