Expression of the midnolin gene is developmentally regulated: it is strongly expressed at the mesencephalon (midbrain) of the embryo in day 12.5 (E12.5) mice. The midnolin encodes a protein of 508 amino acids (aa); which contains a Ubiquitin-like domain. The intracellular distribution of the midnolin was studied by using midnolin-green fluorescent protein (GFP) fusion proteins. Midnolin was found to be localized in the nucleus and nucleolus; but not in the cytoplasm. The nucleolar localization sigl was determined to be a 28aa peptide located at the C-termil region of the midnolin. May be involved in regulation of genes related to neurogenesis in the nucleolus
KLH conjugated synthetic peptide derived from human MIDN 51-150/468
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
affinity purified by Protein A
SwissProt: Q504T8 Human
Midbrain nucleolar protein.
Liver (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-Midnolin (ER1913-02) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kD
Observed band size: 49 kD
Publishing research using Anti-Midnolin antibody? Please let us know so
that we can cite the reference in this datasheet.
Choose your product,application and species below,then click
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you
Click here to view the