This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least six highly degenerated leucine-rich repeats. This family member plays a role in epigenetic silencing. It nucleates at CpG islands and specifically demethylates both mono- and di-methylated lysine-36 of histone H3. Alternative splicing results in multiple transcript variants.
Purified recombinant fragment of human KDM2A (AA: 1-150) expressed in E. Coli.
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
1*PBS with 0.05% sodium azide.
Protein G purified.
[Histone-H3]-lysine-36 demethylase 1A antibody
CXXC-type zinc finger protein 8 antibody
F box / LRR repeat protein 11 antibody
F box and leucine rich repeat protein 11 antibody
F box protein FBL7 antibody
F-box and leucine-rich repeat protein 11 antibody
F-box protein FBL7 antibody
F-box protein Lilina antibody
F-box/LRR-repeat protein 11 antibody
JmjC domain-containing histone demethylation protein 1A antibody
Jumonji C domain containing histone demethylase 1A antibody
Lysine (K) specific demethylase 2A antibody
Lysine-specific demethylase 2A antibody
Fig1: Western blot analysis of KDM2A against human KDM2A (AA: 1-150) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Western blot analysis of KDM2A against HEK293 (1) and KDM2A (AA: 1-150)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1712-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.
1. Mol Cancer Res. 2018 Jan;16(1):58-68.
2. Cancer Biomark. 2017 Jul 19;20(1):23-29.
Publishing research using KDM2A? Please let us know so
that we can cite the reference in this datasheet.
Choose your product,application and species below,then click
On the next page you can complete and submit your review.
Leave us a review
To our knowledge, customised protocols are not required for this product.
Please try the standard protocols listed below and let us know how you
Click here to view the