CD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
Immunogen affinity purified
CD34 antigen antibody
CD34 molecule antibody
Cluster designation 34 antibody
Hematopoietic progenitor cell antigen CD34 antibody
Fig1: Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1000 dilution.
Lane 1: Human brain
Lane 2: Mouse brain
Lane 3: Mouse testis
Lane 4: Mouse thymus
Lane 5: Mouse spleen
Lane 7: TF-1
Lane 8: F9
Fig2: ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- CD34 rabbit polyclonal antibody.
1. Freund D., Wiebe G.J., Ehninger G., Corbeil D.; "Sequence analysis of the human CD34 antigen."; Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.
2. The MGC Project Team;"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
3. Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E.;"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.";Leukemia 2:793-803(1988).
Publishing research using Anti-CD34 antibody? Please let us know so
that we can cite the reference in this datasheet.
Gu, B., Zhang, J., Wu, Y., Zhang, X., Tan, Z., Lin, Y., . . . Zhang, M. (2011). Proteomic analyses reveal common promiscuous patterns of cell surface proteins on human embryonic stem cells and sperms. PLoS One, 6(5), e19386.
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